ArticleScientific reports2016
A common mechanism involving the TORC1 pathway can lead to amphotericin B-persistence in biofilm and planktonic Saccharomyces cerevisiae populations.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- [Chlorhexidine and Polident Induce the Formation ofSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2026Article
- Echinocandin tolerance and persistencemBio · 2026Article
- Persister cells in human fungal pathogens.PLoS pathogens · 2025Review
- Antifungal persistence: Clinical relevance and mechanisms.PLoS pathogens · 2025Review
- The effect of copper on the antifungal activity of polyethyleneimines against quiescent conidia and germlings of Aspergillus nidulans.Scientific reports · 2025Article
- Insights into the role of sterol metabolism in antifungal drug resistance: a mini-review.Frontiers in microbiology · 2024Review
- Common virulence factors betweenCurrent research in microbial sciences · 2024Review
- The continuing evolution of barcode applications: Functional toxicology to cell lineage.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- Targeting Virulence Factors ofFoods (Basel, Switzerland) · 2022Review
- What 'Omics can tell us about antifungal adaptation.FEMS yeast research · 2022Review
- Genomic and Targeted Approaches Unveil the Cell Membrane as a Major Target of the Antifungal Cytotoxin Amantelide A.Chembiochem : a European journal of chemical biology · 2021Article
- Studying Bacterial Persistence: Established Methods and Current Advances.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Filamentation Regulatory Pathways Control Adhesion-Dependent Surface Responses in Yeast.Genetics · 2019Article
- ABC Transporter Genes Show Upregulated Expression in Drug-Resistant Clinical Isolates ofFrontiers in microbiology · 2019Article
- Fungal persister cells: The basis for recalcitrant infections?PLoS pathogens · 2018Review
- Gaining Insights from Candida Biofilm Heterogeneity: One Size Does Not Fit All.Journal of fungi (Basel, Switzerland) · 2018Review
- Article
- Candida glabrata Biofilms: How Far Have We Come?Journal of fungi (Basel, Switzerland) · 2017Review
- Persistence and drug tolerance in pathogenic yeast.Current genetics · 2017Review
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Fungal infections are an increasing clinical problem. Decreased treatment effectiveness is associated with biofilm formation and drug recalcitrance is thought to be biofilm specific. However, no systematic investigations have tested whether resistance mechanisms are shared between biofilm and planktonic populations. We performed multiplexed barcode sequencing (Bar-seq) screening of a pooled collection of gene-deletion mutants cultivated as biofilm and planktonic cells. Screening for resistance to the ergosterol-targeting fungicide amphotericin B (AmB) revealed that the two growth modes had significant overlap in AmB-persistent mutants. Mutants defective in sterol metabolism, ribosome biosynthesis, and the TORC1 and Ras pathways showed increased persistence when treated with AmB. The ras1, ras2 and tor1 mutants had a high-persister phenotype similar to wild-type biofilm and planktonic cells exposed to the TORC1 pathway inhibitor rapamycin. Inhibition of TORC1 with rapamycin also increased the proportion of persisters in Candida albicans and Candida glabrata. We propose that decreased TORC1-mediated induction of ribosome biosynthesis via Ras can lead to formation of AmB-persister cells regardless of whether the cells are in planktonic or biofilm growth mode. Identification of common pathways leading to growth mode-independent persister formation is important for developing novel strategies for treating fungal infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.