ArticleEnvironmental science and pollution research international2017
Substrate-independent luminescent phage-based biosensor to specifically detect enteric bacteria such as E. coli.
Article in Environmental science and pollution research international, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Engineering Phages to Fight Multidrug-Resistant Bacteria.Chemical reviews · 2025Review
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment.Biosensors · 2022Review
- The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics.Antibiotics (Basel, Switzerland) · 2022Review
- Luminescent Phage-Based Detection ofPharmaceuticals (Basel, Switzerland) · 2021Article
- Reporter Phage-Based Detection of Bacterial Pathogens: Design Guidelines and Recent Developments.Viruses · 2020Review
- Review
- Epigenetic biosensors for bacteriophage detection and phage receptor discrimination.Environmental microbiology · 2020Article
- Methods for detection of viable foodborne pathogens: current state-of-art and future prospects.Applied microbiology and biotechnology · 2020Review
- Rebooting Synthetic Phage-Inducible Chromosomal Islands: One Method to Forge Them All.Biodesign research · 2020Article
- Bacteriophages as Potential Tools for Detection and Control ofMicroorganisms · 2019Review
- Multifunctional Nanotechnology-Enabled Sensors for Rapid Capture and Detection of Pathogens.Sensors (Basel, Switzerland) · 2017Review
- Engineering of Bacteriophages Y2::Applied and environmental microbiology · 2017Article
- Semi-autonomous inline water analyzer: design of a common light detector for bacterial, phage, and immunological biosensors.Environmental science and pollution research international · 2017Article
- The Use of a Novel NanoLuc -Based Reporter Phage for the Detection of Escherichia coli O157:H7.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Water quality is a major safety consideration in environments that are impacted by human activity. The key challenge of the COMBITOX project is to develop a unique instrument that can accommodate several biodetector systems (see the accompanying COMBITOX papers) able to detect different pollutants such as bacteria, toxins, and heavy metals. The output signal chosen by our consortium is based on luminescence detection. Our group recently developed phage-based biosensors using gfp as a reporter gene to detect enteric bacteria in complex environments such as sea water, and the main challenge we faced was to adapt our biodetector to a luminescent signal that could fit the COMBITOX project requirements. Another key point was to use a substrate-independent reporter system in order to avoid substrate addition in the detection prototype. This paper describes the development of a phage-based biodetector using a luminescent and substrate-independent output to detect some enteric bacteria, such as Escherichia coli, in water samples. We have successfully engineered various prototypes using the HK620 and HK97 bacteriophages that use different packaging systems, and both proved functional for the integration of the full luxCDABE operon controlled by two different bacterial promoters. We show that the luxCDABE operon controlled by the PrplU bacterial promoter is the most efficient in terms of signal emission. The emission of luminescence is specific and allows the detection of 10
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