Evidence map›Paper›PMID 26902619›Full record

ArticleScientific reports2016

Living biointerfaces based on non-pathogenic bacteria support stem cell differentiation.

Jake J Hay, Aleixandre Rodrigo-Navarro, Karoliina Hassi, Vladimira Moulisova, Matthew J Dalby, Manuel Salmeron-Sanchez

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 20 citations in OpenAlex.

  1. Probiotic-Based Materials as Living Therapeutics.Advanced materials (Deerfield Beach, Fla.) · 2026
    Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Toward Light-Regulated Living Biomaterials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jake J HayDivision of Biomedical Engineering, School of Engineering, University of Glasgow, UK.
Aleixandre Rodrigo-NavarroDivision of Biomedical Engineering, School of Engineering, University of Glasgow, UK.
Karoliina HassiDivision of Biomedical Engineering, School of Engineering, University of Glasgow, UK.
Vladimira MoulisovaDivision of Biomedical Engineering, School of Engineering, University of Glasgow, UK.
Matthew J DalbyCentre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, University of Glasgow, UK.
Manuel Salmeron-SanchezDivision of Biomedical Engineering, School of Engineering, University of Glasgow, UK.
University of Glasgow · GB

Funding

Biotechnology and Biological Sciences Research Council BB/G008868/1Biotechnology and Biological Sciences Research Council JF20604
6 · The paper itself

Abstract

Lactococcus lactis, a non-pathogenic bacteria, has been genetically engineered to express the III7-10 fragment of human fibronectin as a membrane protein. The engineered L. lactis is able to develop biofilms on different surfaces (such as glass and synthetic polymers) and serves as a long-term substrate for mammalian cell culture, specifically human mesenchymal stem cells (hMSC). This system constitutes a living interface between biomaterials and stem cells. The engineered biofilms remain stable and viable for up to 28 days while the expressed fibronectin fragment induces hMSC adhesion. We have optimised conditions to allow long-term mammalian cell culture, and found that the biofilm is functionally equivalent to a fibronectin-coated surface in terms of osteoblastic differentiation using bone morphogenetic protein 2 (BMP-2) added to the medium. This living bacteria interface holds promise as a dynamic substrate for stem cell differentiation that can be further engineered to express other biochemical cues to control hMSC differentiation.

Indexed as

Acrylic ResinsBiofilmsBone Morphogenetic Protein 2Cell AdhesionCell DifferentiationCell ProliferationCoated Materials, BiocompatibleFibronectinsGene ExpressionGenetic EngineeringGlassHumansLactococcus lactisMesenchymal Stem CellsOsteoblastsPeptidesAcrylic ResinsBMP2 protein, humanBone Morphogenetic Protein 2Coated Materials, BiocompatibleFibronectinsPeptidespoly(ethylacrylate)

Identifiers

PMID26902619
PMCPMC4763179
OpenAlexW2309657216

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.