Evidence map›Paper›PMID 26902610›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2016

Interplay of the physical microenvironment, contact guidance, and intracellular signaling in cell decision making.

Colin D Paul, Daniel J Shea, Megan R Mahoney, Andreas Chai, Victoria Laney, Wei-Chien Hung, Konstantinos Konstantopoulos

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
4.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 51 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Cell-Taxi: Mesenchymal Cells Carry and Transport Clusters of Cancer Cells.Small (Weinheim an der Bergstrasse, Germany) · 2022
    Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Colin D PaulDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA; Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore, Maryland, USA; and.
Daniel J SheaDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA;
Megan R MahoneyDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA;
Andreas ChaiDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA;
Victoria LaneyDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA;
Wei-Chien HungDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA;
Konstantinos KonstantopoulosDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA; Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore, Maryland, USA; and Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, Maryland, USA konstant@jhu.edu.
Johns Hopkins University · USJohns Hopkins Medicine · US

Funding

Development of high throughput screening technologies in breast cancerR01CA183804 · NCI · JOHNS HOPKINS UNIVERSITY · PI KONSTANTOPOULOS, KONSTANTINOS, KONTROGIANNI-KONSTANTOPOULOS, AIKATERINI · 2015 to 2019
$3.5M
Pancreatic Cancer Cell Mechanics and ImagingR01CA186286 · NCI · JOHNS HOPKINS UNIVERSITY · PI KONSTANTOPOULOS, KONSTANTINOS · 2014 to 2018
$2.1M
NCI NIH HHS R01 CA183804NCI NIH HHS R01 CA186286
6 · The paper itself

Abstract

The peritumoral physical microenvironment consists of complex topographies that influence cell migration. Cell decision making, upon encountering anisotropic, physiologically relevant physical cues, has yet to be elucidated. By integrating microfabrication with cell and molecular biology techniques, we provide a quantitative and mechanistic analysis of cell decision making in a variety of well-defined physical microenvironments. We used MDA-MB-231 breast carcinoma and HT1080 fibrosarcoma as cell models. Cell decision making after lateral confinement in 2-dimensional microcontact printed lines is governed by branch width at bifurcations. Cells confined in narrow feeder microchannels prefer to enter wider branches at bifurcations. In contrast, in feeder channels that are wider than the cell body, cells elongate along one side wall of the channel and are guided by contact with the wall to the contiguous branch channel independent of its width. Knockdown of β1-integrins or inhibition of cellular contractility suppresses contact guidance. Concurrent, but not individual, knockdown of nonmuscle myosin isoforms IIA and IIB also decreases contact guidance, which suggests the existence of a compensatory mechanism between myosin IIA and myosin IIB. Conversely, knockdown or inhibition of cell division control protein 42 homolog promotes contact guidance-mediated decision making. Taken together, the dimensionality, length scales of the physical microenvironment, and intrinsic cell signaling regulate cell decision making at intersections.-Paul, C. D., Shea, D. J., Mahoney, M. R., Chai, A., Laney, V., Hung, W.-C., Konstantopoulos, K. Interplay of the physical microenvironment, contact guidance, and intracellular signaling in cell decision making.

Indexed as

Cellular MicroenvironmentBreast Neoplasmscdc42 GTP-Binding ProteinCell Line, TumorCell MovementCulture MediaFemaleFibrosarcomaGene Expression RegulationHumansMicrofluidicsSignal Transductioncdc42 GTP-Binding ProteinCulture Mediacell migrationconfinementmicrofluidics

Identifiers

PMID26902610
PMCPMC4871803
OpenAlexW2294596645

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.