ArticleScientific reports2016
High-resolution Identification and Separation of Living Cell Types by Multiple microRNA-responsive Synthetic mRNAs.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Split RNA switch orchestrates pre- and post-translational control to enable cell type-specific gene expression.Nature communications · 2025Article
- Generation and purification of iPSC-derived cardiomyocytes for clinical applications.Stem cell research & therapy · 2025Article
- Synthetic circular RNA switches and circuits that control protein expression in mammalian cells.Nucleic acids research · 2023Article
- Sensing intracellular signatures with synthetic mRNAs.RNA biology · 2023Review
- Purification of human iPSC-derived cells at large scale using microRNA switch and magnetic-activated cell sorting.Stem cell reports · 2022Article
- A versatile and robust cell purification system with an RNA-only circuit composed of microRNA-responsive ON and OFF switches.Science advances · 2022Article
- N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells.Nucleic acids research · 2020Article
- Caliciviral protein-based artificial translational activator for mammalian gene circuits with RNA-only delivery.Nature communications · 2020Article
- Numerical operations in living cells by programmable RNA devices.Science advances · 2019Article
- MicroRNA-Based Separation of Cortico-Fugal Projection Neuron-Like Cells Derived From Embryonic Stem Cells.Frontiers in neuroscience · 2019Article
- Synthetic RNA-based logic computation in mammalian cells.Nature communications · 2018Article
- An update on stem cell biology and engineering for brain development.Molecular psychiatry · 2017Review
- Dormant Pluripotent Cells Emerge during Neural Differentiation of Embryonic Stem Cells in a FoxO3-Dependent Manner.Molecular and cellular biology · 2017Article
- MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells.Scientific reports · 2016Article
- RNA-based gene circuits for cell regulation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2016Review
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The precise identification and separation of living cell types is critical to both study cell function and prepare cells for medical applications. However, intracellular information to distinguish live cells remains largely inaccessible. Here, we develop a method for high-resolution identification and separation of cell types by quantifying multiple microRNA (miRNA) activities in live cell populations. We found that a set of miRNA-responsive, in vitro synthesized mRNAs identify a specific cell population as a sharp peak and clearly separate different cell types based on less than two-fold differences in miRNA activities. Increasing the number of miRNA-responsive mRNAs enhanced the capability for cell identification and separation, as we precisely and simultaneously distinguished different cell types with similar miRNA profiles. In addition, the set of synthetic mRNAs separated HeLa cells into subgroups, uncovering heterogeneity of the cells and the level of resolution achievable. Our method could identify target live cells and improve the efficiency of cell purification from heterogeneous populations.
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