ArticleScientific reports2016
Bioconversion of methane to lactate by an obligate methanotrophic bacterium.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 158 citations in OpenAlex.
- Evading native restriction-modification systems improves electroporation efficiency of the methanotrophApplied and environmental microbiology · 2026Article
- Methanoperedenaceae archaea: a 20-year research journey.Nature communications · 2026Review
- Biological conversion of methane to organic molecules: towards a low-carbon bioeconomy.National science review · 2026Review
- Enhancing D-lactic acid production from methane through metabolic engineering of Methylomonas sp. DH-1.Microbial cell factories · 2025Article
- Advancements in the production of value-added products via methane biotransformation by methanotrophs: Current status and future perspectives.Journal of microbiology (Seoul, Korea) · 2025Review
- Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1.Microbial cell factories · 2024Article
- Overexpression of native carbonic anhydrases increases carbon conversion efficiency in the methanotrophic biocatalystmSphere · 2024Article
- Construction of a broad-host-range Anderson promoter series and particulate methane monooxygenase promoter variants expand the methanotroph genetic toolbox.Synthetic and systems biotechnology · 2024Article
- Imbalanced metabolism induced NHInternational microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- A synthetic co-culture for bioproduction of ammonia from methane and air.Journal of industrial microbiology & biotechnology · 2024Article
- Optimization of electroporation method and promoter evaluation for type-1 methanotroph,Frontiers in bioengineering and biotechnology · 2024Article
- All Kinds of Sunny Colors Synthesized from Methane: Genome-Encoded Carotenoid Production byMicroorganisms · 2023Article
- Regulation of acetate tolerance by small ORF-encoded polypeptides modulating efflux pump specificity in Methylomonas sp. DH-1.Biotechnology for biofuels and bioproducts · 2023Article
- Cre/Applied and environmental microbiology · 2023Article
- Transcription regulation strategies in methylotrophs: progress and challenges.Bioresources and bioprocessing · 2022Review
- Adaptive evolution of Methylotuvimicrobium alcaliphilum to grow in the presence of rhamnolipids improves fatty acid and rhamnolipid production from CH4.Journal of industrial microbiology & biotechnology · 2022Article
- Optimized Tools and Methods for Methanotroph Genome Editing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients.Frontiers in bioengineering and biotechnology · 2022Review
- Systems Metabolic Engineering of Methanotrophic Bacteria for Biological Conversion of Methane to Value-Added Compounds.Advances in biochemical engineering/biotechnology · 2022Article
- Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RubisCO) Is Essential for Growth of the Methanotroph Methylococcus capsulatus Strain Bath.Applied and environmental microbiology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methane is the second most abundant greenhouse gas (GHG), with nearly 60% of emissions derived from anthropogenic sources. Microbial conversion of methane to fuels and value-added chemicals offers a means to reduce GHG emissions, while also valorizing this otherwise squandered high-volume, high-energy gas. However, to date, advances in methane biocatalysis have been constrained by the low-productivity and limited genetic tractability of natural methane-consuming microbes. Here, leveraging recent identification of a novel, tractable methanotrophic bacterium, Methylomicrobium buryatense, we demonstrate microbial biocatalysis of methane to lactate, an industrial platform chemical. Heterologous overexpression of a Lactobacillus helveticus L-lactate dehydrogenase in M. buryatense resulted in an initial titer of 0.06 g lactate/L from methane. Cultivation in a 5 L continuously stirred tank bioreactor enabled production of 0.8 g lactate/L, representing a 13-fold improvement compared to the initial titer. The yields (0.05 g lactate/g methane) and productivity (0.008 g lactate/L/h) indicate the need and opportunity for future strain improvement. Additionally, real-time analysis of methane utilization implicated gas-to-liquid transfer and/or microbial methane consumption as process limitations. This work opens the door to develop an array of methanotrophic bacterial strain-engineering strategies currently employed for biocatalytic sugar upgrading to "green" chemicals and fuels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.