ArticleScientific reports2016
A novel low-power laser-mediated transfer of foreign molecules into cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
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- Dynamics of Actin Cytoskeleton and Their Signaling Pathways during Cellular Wound Repair.Cells · 2022Article
- Cell behaviors within a confined adhesive area fabricated using novel micropatterning methods.PloS one · 2022Article
- Deletion of gmfA induces keratocyte-like migration in Dictyostelium.FEBS open bio · 2022Article
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- CaCells · 2020Review
- Regulation of the Total Cell Surface Area in DividingFrontiers in cell and developmental biology · 2020Article
- Dynamin-Like Protein B ofCells · 2019Article
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- Manipulation of cell migration by laserporation-induced local wounding.Scientific reports · 2019Article
- A study of wound repair in Dictyostelium cells by using novel laserporation.Scientific reports · 2018Article
- Effect of Laser Irradiation on Cell Cycle and Mitosis.Journal of lasers in medical sciences · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficiently introducing molecules such as chemical drugs, proteins, or nucleic acids into cells is a central technique in cell and molecular biology, gene therapy and regenerative medicine. The cell membrane is a critical barrier for this purpose. While many approaches exist, some of which are applicable to single cells that researchers specify under microscopy, no reliable and efficient technique has been invented. In this study, cells were cultured on a coverslip that had been coated with carbon by vapor deposition, and a laser beam was focused on a small local spot beneath a single cell under microscopy. The absorbed energy of the laser beam by the carbon made a pore only in the cell membrane that was attached to the carbon coat, which resulted in an efficient introduction. An inexpensive and lower-power laser could be used for this method, and the introduction efficiency was 100% without any loss of cell viability. This new technique will provide a powerful tool not only to research but also to many applied fields.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.