ArticleNature communications2016
An interactive web-based application for Comprehensive Analysis of RNAi-screen Data.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- nf-core/crisprseq: a versatile pipeline for comprehensive analysis of CRISPR gene editing and screening assays.NAR genomics and bioinformatics · 2026Article
- Image-Based Screening for Stress Granule Regulators.Methods in molecular biology (Clifton, N.J.) · 2022Article
- SIGNAL: A web-based iterative analysis platform integrating pathway and network approaches optimizes hit selection from genome-scale assays.Cell systems · 2021Article
- Beta-binomial modeling of CRISPR pooled screen data identifies target genes with greater sensitivity and fewer false negatives.Genome research · 2019Article
- A Complementary Chemical and Genomic Screening Approach for Druggable Targets in the Nrf2 Pathway and Small Molecule Inhibitors to Overcome Cancer Cell Drug Resistance.ACS chemical biology · 2018Article
- RNA Interference (RNAi) Screening inGenetics · 2018Article
- HTSvis: a web app for exploratory data analysis and visualization of arrayed high-throughput screens.Bioinformatics (Oxford, England) · 2017Article
- Genome-wide siRNA screen of genes regulating the LPS-induced NF-κB and TNF-α responses in mouse macrophages.Scientific data · 2017Article
- Genome-wide siRNA screen of genes regulating the LPS-induced TNF-α response in human macrophages.Scientific data · 2017Article
- HTS-Net: An integrated regulome-interactome approach for establishing network regulation models in high-throughput screenings.PloS one · 2017Article
Corrections and comments
- Erratum issuedCorrigendum: An interactive web-based application for Comprehensive Analysis of RNAi-screen Data.2016 · Beuhler, Eugen [Corrected to Buehler, Eugen]
Authors and funding
13 authors.
Funding
Abstract
RNAi screens are widely used in functional genomics. Although the screen data can be susceptible to a number of experimental biases, many of these can be corrected by computational analysis. For this purpose, here we have developed a web-based platform for integrated analysis and visualization of RNAi screen data named CARD (for Comprehensive Analysis of RNAi Data; available at https://card.niaid.nih.gov). CARD allows the user to seamlessly carry out sequential steps in a rigorous data analysis workflow, including normalization, off-target analysis, integration of gene expression data, optimal thresholds for hit selection and network/pathway analysis. To evaluate the utility of CARD, we describe analysis of three genome-scale siRNA screens and demonstrate: (i) a significant increase both in selection of subsequently validated hits and in rejection of false positives, (ii) an increased overlap of hits from independent screens of the same biology and (iii) insight to microRNA (miRNA) activity based on siRNA seed enrichment.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.