ArticleEpigenetics2016
Analysis of chromatin structure in mouse preimplantation embryos by fluorescent recovery after photobleaching.
Article in Epigenetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 52 citations in OpenAlex.
- Expression of LINE-1 elements is required for preimplantation development and totipotency.Genes & diseases · 2025Review
- Embryonic Origins of Cancer: Insights from Double Homeobox 4 Regulation.Biomolecules · 2025Review
- H3.1/3.2 regulate the initial progression of the gene expression program.Nucleic acids research · 2024Article
- Chromatin structure in totipotent mouse early preimplantation embryos.The Journal of reproduction and development · 2024Review
- Involvement of H2A variants in DNA damage response of zygotes.Cell death discovery · 2024Article
- H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency.Stem cell reports · 2024Article
- Advances in understanding the regulation of pluripotency fate transition in embryonic stem cells.Frontiers in cell and developmental biology · 2024Review
- Dynamic nucleosome remodeling mediated by YY1 underlies early mouse development.Genes & development · 2023Article
- A change in biophysical properties accompanies heterochromatin formation in mouse embryos.Genes & development · 2023Article
- Histone remodeling reflects conserved mechanisms of bovine and human preimplantation development.EMBO reports · 2023Article
- The Oocyte-Specific Linker Histone H1FOO Is Not Essential for Mouse Oogenesis and Fertility.Cells · 2022Article
- 2-Cell-like Cells: An Avenue for Improving SCNT Efficiency.Biomolecules · 2022Review
- Paternally inherited H3K27me3 affects chromatin accessibility in mouse embryos produced by round spermatid injection.Development (Cambridge, England) · 2022Article
- Parental competition for the regulators of chromatin dynamics in mouse zygotes.Communications biology · 2022Article
- Epigenetic Reprogramming in Early Animal Development.Cold Spring Harbor perspectives in biology · 2022Review
- Zygotic gene activation in mice: profile and regulation.The Journal of reproduction and development · 2022Review
- Regulation of paternal 5mC oxidation and H3K9me2 asymmetry by ERK1/2 in mouse zygotes.Cell & bioscience · 2022Article
- Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events.BMC research notes · 2021Article
- H3.3 kinetics predicts chromatin compaction status of parental genomes in early embryos.Reproductive biology and endocrinology : RB&E · 2021Article
- Functions and Regulation of Endogenous Retrovirus Elements during Zygotic Genome Activation: Implications for Improving Somatic Cell Nuclear Transfer Efficiency.Biomolecules · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zygotes are totipotent cells that have the ability to differentiate into all cell types. It is believed that this ability is lost gradually and differentiation occurs along with the progression of preimplantation development. Here, we hypothesized that the loose chromatin structure is involved in the totipotency of one-cell stage embryos and that the change from loose to tight chromatin structure is associated with the loss of totipotency. To address this hypothesis, we investigated the mobility of eGFP-tagged histone H2B (eGFP-H2B), which is an index for the looseness of chromatin, during preimplantation development based on fluorescent recovery after photobleaching (FRAP) analysis. The highest mobility of eGFP-H2B was observed in pronuclei in 1-cell stage embryos and mobility gradually decreased during preimplantation development. The decrease in mobility between the 1- and 2-cell stages depended on DNA synthesis in 2-cell stage embryos. In nuclear transferred embryos, chromatin in the pseudopronuclei loosened to a level comparable to the pronuclei in 1-cell stage embryos. These results indicated that the mobility of eGFP-H2B is negatively correlated with the degree of differentiation of preimplantation embryos. Therefore, we suggest that highly loosened chromatin is involved in totipotency of 1-cell embryos and the loss of looseness is associated with differentiation during preimplantation development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.