ArticlePloS one2016
Synthetic Transcription Amplifier System for Orthogonal Control of Gene Expression in Saccharomyces cerevisiae.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Tailoring Corynebacterium glutamicum for Sustainable Biomanufacturing: From Traditional to Cutting-Edge Technologies.Molecular biotechnology · 2026Review
- Expanding the Yeast MoClo Toolkit: gene expression control parts forSynthetic biology (Oxford, England) · 2025Article
- A universal system for boosting gene expression in eukaryotic cell-lines.Nature communications · 2024Article
- Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit.Nature communications · 2024Article
- Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol.Scientific reports · 2022Article
- Control of D-lactic acid content in P(LA-3HB) copolymer in the yeastMetabolic engineering communications · 2022Article
- Review
- Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae.FEMS yeast research · 2021Article
- Review
- Genetic circuit design automation for yeast.Nature microbiology · 2020Article
- Synthetic control devices for gene regulation in Penicillium chrysogenum.Microbial cell factories · 2019Article
- Yeast Expression Systems: Overview and Recent Advances.Molecular biotechnology · 2019Review
- Dynamic control of endogenous metabolism with combinatorial logic circuits.Molecular systems biology · 2018Article
- A universal gene expression system for fungi.Nucleic acids research · 2018Article
- Synthetic Toolkit for Complex Genetic Circuit Engineering in Saccharomyces cerevisiae.ACS synthetic biology · 2018Article
- A combinatorial approach to synthetic transcription factor-promoter combinations for yeast strain engineering.Yeast (Chichester, England) · 2018Article
- The Genetic Makeup and Expression of the Glycolytic and Fermentative Pathways Are Highly Conserved Within theFrontiers in genetics · 2018Article
- Synthetic Promoters and Transcription Factors for Heterologous Protein Expression inFrontiers in bioengineering and biotechnology · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
This work describes the development and characterization of a modular synthetic expression system that provides a broad range of adjustable and predictable expression levels in S. cerevisiae. The system works as a fixed-gain transcription amplifier, where the input signal is transferred via a synthetic transcription factor (sTF) onto a synthetic promoter, containing a defined core promoter, generating a transcription output signal. The system activation is based on the bacterial LexA-DNA-binding domain, a set of modified, modular LexA-binding sites and a selection of transcription activation domains. We show both experimentally and computationally that the tuning of the system is achieved through the selection of three separate modules, each of which enables an adjustable output signal: 1) the transcription-activation domain of the sTF, 2) the binding-site modules in the output promoter, and 3) the core promoter modules which define the transcription initiation site in the output promoter. The system has a novel bidirectional architecture that enables generation of compact, yet versatile expression modules for multiple genes with highly diversified expression levels ranging from negligible to very strong using one synthetic transcription factor. In contrast to most existing modular gene expression regulation systems, the present system is independent from externally added compounds. Furthermore, the established system was minimally affected by the several tested growth conditions. These features suggest that it can be highly useful in large scale biotechnology applications.
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