ArticleInternational journal of molecular sciences2016
Deep Sequencing and Screening of Differentially Expressed MicroRNAs Related to Milk Fat Metabolism in Bovine Primary Mammary Epithelial Cells.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Construction of the ceRNA Regulatory Network Associated with Milk Fat Metabolism.Animals : an open access journal from MDPI · 2026Article
- Functional analysis and identification of miRNAs associated with lipid metabolism from milk-derived exosomes.Journal of animal science and biotechnology · 2026Article
- Integrating miRNAs with QTL for identification of milk and reproduction related traits in dairy cattle.Genes & genomics · 2025Article
- Effects of Genetic Polymorphism in theAnimals : an open access journal from MDPI · 2024Article
- The Complement Component 4 Binding Protein α Gene: A Versatile Immune Gene That Influences Lipid Metabolism in Bovine Mammary Epithelial Cell Lines.International journal of molecular sciences · 2024Article
- miR-497 RegulatesGenes · 2023Article
- Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells.Animals : an open access journal from MDPI · 2022Article
- Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.BMC genomics · 2022Article
- Screening and Conjoint Analysis of Key lncRNAs for Milk Fat Metabolism in Dairy Cows.Frontiers in genetics · 2022Article
- Bta-miR-33a affects gene expression and lipid levels in Chinese Holstein mammary epithelial cells.Archives animal breeding · 2022Article
- The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep.Non-coding RNA · 2021Review
- First DNA Sequencing in Beninese Indigenous Cattle Breeds Captures New Milk Protein Variants.Genes · 2021Article
- In silico identification of variations in microRNAs with a potential impact on dairy traits using whole ruminant genome SNP datasets.Scientific reports · 2021Article
- Integrative analysis of miRNAs and mRNAs revealed regulation of lipid metabolism in dairy cattle.Functional & integrative genomics · 2021Article
- Review
- Effects of polymorphism of theArchives animal breeding · 2021Article
- MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells.Animals : an open access journal from MDPI · 2020Article
- Comprehensive MicroRNA Expression Profile of the Mammary Gland in Lactating Dairy Cows With Extremely Different Milk Protein and Fat Percentages.Frontiers in genetics · 2020Article
- Bta-miR-34b regulates milk fat biosynthesis by targeting mRNA decapping enzyme 1A (DCP1A) in cultured bovine mammary epithelial cells1.Journal of animal science · 2019Article
- RumimiR: a detailed microRNA database focused on ruminant species.Database : the journal of biological databases and curation · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Milk fat is a key factor affecting milk quality and is also a major trait targeted in dairy cow breeding. To determine how the synthesis and the metabolism of lipids in bovine milk is regulated at the miRNA level, primary mammary epithelial cells (pMEC) derived from two Chinese Holstein dairy cows that produced extreme differences in milk fat percentage were cultured by the method of tissue nubbles culture. Small RNA libraries were constructed from each of the two pMEC groups, and Solexa sequencing and bioinformatics analysis were then used to determine the abundance of miRNAs and their differential expression pattern between pMECs. Target genes and functional prediction of differentially expressed miRNAs by Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes analysis illustrated their roles in milk fat metabolism. Results show that a total of 292 known miRNAs and 116 novel miRNAs were detected in both pMECs. Identification of known and novel miRNA candidates demonstrated the feasibility and sensitivity of sequencing at the cellular level. Additionally, 97 miRNAs were significantly differentially expressed between the pMECs. Finally, three miRNAs including bta-miR-33a, bta-miR-152 and bta-miR-224 whose predicted target genes were annotated to the pathway of lipid metabolism were screened and verified by real-time qPCR and Western-blotting experiments. This study is the first comparative profiling of the miRNA transcriptome in pMECs that produce different milk fat content.
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