ArticlePloS one2016
Intravital Fluorescence Excitation in Whole-Animal Optical Imaging.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Age-related changes in lysosomal abundance in mouse hearts assessed by Lysotracker fluorescence imaging and autophagy gene expression analysis.The international journal of cardiovascular imaging · 2026Article
- Review
- In vivo fluorescence imaging: success in preclinical imaging paves the way for clinical applications.Journal of nanobiotechnology · 2022Review
- Rapid Tuberculosis Diagnosis Using Reporter Enzyme Fluorescence.Journal of clinical microbiology · 2019Article
- Fluorescence modeling ofBiomedical optics express · 2019Article
- Monitoring Tuberculosis Drug Activity in Live Animals by Near-Infrared Fluorescence Imaging.Antimicrobial agents and chemotherapy · 2019Article
- Topically Applied Curcumin-Loaded Nanoparticles Treat Erectile Dysfunction in a Rat Model of Type-2 Diabetes.The journal of sexual medicine · 2018Article
- Optical model of the murine lung to optimize pulmonary illumination.Journal of biomedical optics · 2018Article
- Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence.Journal of visualized experiments : JoVE · 2018Article
- Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure.Journal of visualized experiments : JoVE · 2018Article
- Intravital excitation increases detection sensitivity for pulmonary tuberculosis by whole-body imaging with β-lactamase reporter enzyme fluorescence.Journal of biophotonics · 2017Article
- Formulation of image fusion as a constrained least squares optimization problem.Journal of medical imaging (Bellingham, Wash.) · 2017Article
- New directions using reporter enzyme fluorescence (REF) as a tuberculosis diagnostic platform.Tuberculosis (Edinburgh, Scotland) · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Whole-animal fluorescence imaging with recombinant or fluorescently-tagged pathogens or cells enables real-time analysis of disease progression and treatment response in live animals. Tissue absorption limits penetration of fluorescence excitation light, particularly in the visible wavelength range, resulting in reduced sensitivity to deep targets. Here, we demonstrate the use of an optical fiber bundle to deliver light into the mouse lung to excite fluorescent bacteria, circumventing tissue absorption of excitation light in whole-animal imaging. We present the use of this technology to improve detection of recombinant reporter strains of tdTomato-expressing Mycobacterium bovis BCG (Bacillus Calmette Guerin) bacteria in the mouse lung. A microendoscope was integrated into a whole-animal fluorescence imager to enable intravital excitation in the mouse lung with whole-animal detection. Using this technique, the threshold of detection was measured as 103 colony forming units (CFU) during pulmonary infection. In comparison, the threshold of detection for whole-animal fluorescence imaging using standard epi-illumination was greater than 106 CFU.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.