ArticlePLoS neglected tropical diseases2016
A Molecular Method to Discriminate between Mass-Reared Sterile and Wild Tsetse Flies during Eradication Programmes That Have a Sterile Insect Technique Component.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Near-infrared spectroscopy discriminates mass-reared sterile and wild tsetse flies.PLoS neglected tropical diseases · 2025Article
- Effectiveness of a New Self-Marking Technique inInsects · 2022Article
- The COMBAT project: controlling and progressively minimizing the burden of vector-borne animal trypanosomosis in Africa.Open research Europe · 2022Article
- Review
- A standardised method of marking male mosquitoes with fluorescent dust.Parasites & vectors · 2020Article
- Insect microbial symbionts as a novel source for biotechnology.World journal of microbiology & biotechnology · 2019Review
- Competitiveness and survival of two strains of Glossina palpalis gambiensis in an urban area of Senegal.PLoS neglected tropical diseases · 2017Article
- Influence of temperature and relative humidity on survival and fecundity of three tsetse strains.Parasites & vectors · 2016Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Government of Senegal has embarked several years ago on a project that aims to eradicate Glossina palpalis gambiensis from the Niayes area. The removal of the animal trypanosomosis would allow the development more efficient livestock production systems. The project was implemented using an area-wide integrated pest management strategy including a sterile insect technique (SIT) component. The released sterile male flies originated from a colony from Burkina Faso. METHODOLOGY/PRINCIPAL
findingsMonitoring the efficacy of the sterile male releases requires the discrimination between wild and sterile male G. p. gambiensis that are sampled in monitoring traps. Before being released, sterile male flies were marked with a fluorescent dye powder. The marking was however not infallible with some sterile flies only slightly marked or some wild flies contaminated with a few dye particles in the monitoring traps. Trapped flies can also be damaged due to predation by ants, making it difficult to discriminate between wild and sterile males using a fluorescence camera and / or a fluorescence microscope. We developed a molecular technique based on the determination of cytochrome oxidase haplotypes of G. p. gambiensis to discriminate between wild and sterile males. DNA was isolated from the head of flies and a portion of the 5' end of the mitochondrial gene cytochrome oxidase I was amplified to be finally sequenced. Our results indicated that all the sterile males from the Burkina Faso colony displayed the same haplotype and systematically differed from wild male flies trapped in Senegal and Burkina Faso. This allowed 100% discrimination between sterile and wild male G. p. gambiensis. CONCLUSIONS/SIGNIFICANCE: This tool might be useful for other tsetse control campaigns with a SIT component in the framework of the Pan-African Tsetse and Trypanosomosis Eradication Campaign (PATTEC) and, more generally, for other vector or insect pest control programs.
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