ArticlePloS one2016
DNA Barcoding Green Microalgae Isolated from Neotropical Inland Waters.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 90 citations in OpenAlex.
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- Cryptic infection of a giant virus in a unicellular green alga.Science (New York, N.Y.) · 2025Article
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- Latent infection of an active giant endogenous virus in a unicellular green alga.bioRxiv : the preprint server for biology · 2024Article
- A domesticated photoautotrophic microbial community as a biofilm model system for analyzing the influence of plastic surfaces on invertebrate grazers in limnic environments.Frontiers in microbiology · 2023Article
- Comparative study of multiple approaches for identifying cultivable microalgae population diversity from freshwater samples.PloS one · 2023Article
- Development of Microalgae Biodiesel: Current Status and Perspectives.Microorganisms · 2022Review
- Microbial community associated with the crustose lichen Rhizocarpon geographicum L. (DC.) living on oceanic seashore: A large source of diversity revealed by using multiple isolation methods.Environmental microbiology reports · 2022Article
- Green algae (Viridiplantae) in sediments from three lakes on Vega Island, Antarctica, assessed using DNA metabarcoding.Molecular biology reports · 2022Article
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- Revision of Coelastrella (Scenedesmaceae, Chlorophyta) and first register of this green coccoid microalga for continental Norway.World journal of microbiology & biotechnology · 2020Article
- Comparison of the Photoautotrophic Growth Regimens ofBiology · 2020Article
- A first view on the unsuspected intragenus diversity of N-glycans in Chlorella microalgae.The Plant journal : for cell and molecular biology · 2020Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study evaluated the feasibility of using the Ribulose Bisphosphate Carboxylase Large subunit gene (rbcL) and the Internal Transcribed Spacers 1 and 2 of the nuclear rDNA (nuITS1 and nuITS2) markers for identifying a very diverse, albeit poorly known group, of green microalgae from neotropical inland waters. Fifty-one freshwater green microalgae strains isolated from Brazil, the largest biodiversity reservoir in the neotropics, were submitted to DNA barcoding. Currently available universal primers for ITS1-5.8S-ITS2 region amplification were sufficient to successfully amplify and sequence 47 (92%) of the samples. On the other hand, new sets of primers had to be designed for rbcL, which allowed 96% of the samples to be sequenced. Thirty-five percent of the strains could be unambiguously identified to the species level based either on nuITS1 or nuITS2 sequences' using barcode gap calculations. nuITS2 Compensatory Base Change (CBC) and ITS1-5.8S-ITS2 region phylogenetic analysis, together with morphological inspection, confirmed the identification accuracy. In contrast, only 6% of the strains could be assigned to the correct species based solely on rbcL sequences. In conclusion, the data presented here indicates that either nuITS1 or nuITS2 are useful markers for DNA barcoding of freshwater green microalgae, with advantage for nuITS2 due to the larger availability of analytical tools and reference barcodes deposited at databases for this marker.
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