ArticlePloS one2016
Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 134 citations in OpenAlex.
- Cord Blood Platelet-Poor Plasma Functionalizes Polycaprolactone Scaffolds for Coupled Vascularization and Osteogenesis in Bone Tissue Engineering.Tissue engineering and regenerative medicine · 2026Article
- Versatility of Collagen as a Natural Biopolymer for Biomedical, Food, and Cosmetic Applications.Current protein & peptide science · 2026Review
- An animal component-free bioprocess for synthesizing 3D human matrix scaffolds using mesenchymal stromal cells.Frontiers in cell and developmental biology · 2026Article
- Article
- Methods for Assessing Scaffold Vascularization with Human Endothelial Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Technology for the formation of engineered microvascular network models and their biomedical applications.Nano convergence · 2024Review
- Use of collagen and auricular cartilage in bioengineering: scaffolds for tissue regeneration.Cell and tissue banking · 2024Article
- 3D-Printed Collagen-Nanocellulose Hybrid Bioscaffolds with Tailored Properties for Tissue Engineering Applications.ACS applied bio materials · 2023Article
- Engineering large and geometrically controlled vascularized nerve tissue in collagen hydrogels to restore large-sized volumetric muscle loss.Biomaterials · 2023Article
- Nanomaterials-incorporated hydrogels for 3D bioprinting technology.Nano convergence · 2023Review
- Novel stereological method for estimation of cell counts in 3D collagen scaffolds.Scientific reports · 2023Article
- Antibacterial Activity and Cytocompatibility of Electrospun PLGA Scaffolds Surface-Modified by Pulsed DC Magnetron Co-Sputtering of Copper and Titanium.Pharmaceutics · 2023Article
- Development of fibroblast/endothelial cell-seeded collagen scaffolds for in vitro prevascularization.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- Barrier Membrane in Regenerative Therapy: A Narrative Review.Membranes · 2022Review
- Avoiding artefacts in MicroCT imaging of collagen scaffolds: Effect of phosphotungstic acid (PTA)-staining and crosslink density.Bioactive materials · 2022Article
- Combination of Polysaccharide Nanofibers Derived from Cellulose and Chitin Promotes the Adhesion, Migration and Proliferation of Mouse Fibroblast Cells.Nanomaterials (Basel, Switzerland) · 2022Article
- Cell-Seeded Biomaterial Scaffolds: The Urgent Need for Unanswered Accelerated Angiogenesis.International journal of nanomedicine · 2022Review
- Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments.Frontiers in bioengineering and biotechnology · 2022Article
- Vascularization strategies in tissue engineering approaches for soft tissue repair.Journal of tissue engineering and regenerative medicine · 2021Review
- Recapitulating Cardiac Structure and Function In Vitro from Simple to Complex Engineering.Micromachines · 2021Review
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we describe a porous 3-dimensional collagen scaffold material that supports capillary formation in vitro, and promotes vascularization when implanted in vivo. Collagen scaffolds were synthesized from type I bovine collagen and have a uniform pore size of 80 μm. In vitro, scaffolds seeded with primary human microvascular endothelial cells suspended in human fibrin gel formed CD31 positive capillary-like structures with clear lumens. In vivo, after subcutaneous implantation in mice, cell-free collagen scaffolds were vascularized by host neovessels, whilst a gradual degradation of the scaffold material occurred over 8 weeks. Collagen scaffolds, impregnated with human fibrinogen gel, were implanted subcutaneously inside a chamber enclosing the femoral vessels in rats. Angiogenic sprouts from the femoral vessels invaded throughout the scaffolds and these degraded completely after 4 weeks. Vascular volume of the resulting constructs was greater than the vascular volume of constructs from chambers implanted with fibrinogen gel alone (42.7±5.0 μL in collagen scaffold vs 22.5±2.3 μL in fibrinogen gel alone; p<0.05, n = 7). In the same model, collagen scaffolds seeded with human adipose-derived stem cells (ASCs) produced greater increases in vascular volume than did cell-free collagen scaffolds (42.9±4.0 μL in collagen scaffold with human ASCs vs 25.7±1.9 μL in collagen scaffold alone; p<0.05, n = 4). In summary, these collagen scaffolds are biocompatible and could be used to grow more robust vascularized tissue engineering grafts with improved the survival of implanted cells. Such scaffolds could also be used as an assay model for studies on angiogenesis, 3-dimensional cell culture, and delivery of growth factors and cells in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.