ArticleAnalytical chemistry2016
Droplet Microfluidic Platform for the Determination of Single-Cell Lactate Release.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- SCO-ph: Microfluidic Dynamic Phenotyping Platform for High-Throughput Screening of Single Cell Acidification.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Unveiling the Potential of Single-Cell Encapsulation in Biomedical Applications: Current Advances and Future Perspectives.Small science · 2024Article
- Irregular Antibody Screening Using a Microdroplet Platform.Biosensors · 2023Article
- Pollution-Free and Highly Sensitive Lactate Detection in Cell Culture Based on a Microfluidic Chip.Micromachines · 2023Article
- Metabolism-Related Gene Expression in Circulating Tumor Cells from Patients with Early Stage Non-Small Cell Lung Cancer.Cancers · 2022Article
- Multifunctional microfluidic chip for cancer diagnosis and treatment.Nanotheranostics · 2021Review
- A DNA-mediated crosslinking strategy to enhance cellular delivery and sensor performance of protein spherical nucleic acids.Chemical science · 2020Article
- Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations Based on Single-Cell Glycolysis.Analytical chemistry · 2020Article
- Article
- Monitoring and external control of pH in microfluidic droplets during microbial culturing.Microbial cell factories · 2020Article
- Recent Progress in Lab-On-a-Chip Systems for the Monitoring of Metabolites for Mammalian and Microbial Cell Research.Sensors (Basel, Switzerland) · 2019Review
- Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology.Micromachines · 2019Review
- High throughput gene expression profiling of yeast colonies with microgel-culture Drop-seq.Lab on a chip · 2019Article
- Sorting by interfacial tension (SIFT): label-free selection of live cells based on single-cell metabolism.Lab on a chip · 2019Article
- Multiplexed Single-Cell Measurements of FDG Uptake and Lactate Release Using Droplet Microfluidics.Technology in cancer research & treatment · 2019Article
- Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment.Journal of the Royal Society, Interface · 2017Review
- Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture.Biomicrofluidics · 2017Review
- Biocompatibility of fluids for multiphase drops-in-drops microfluidics.Biomedical microdevices · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Cancer cells release high levels of lactate that has been correlated to increased metastasis and tumor recurrence. Single-cell measurements of lactate release can identify malignant cells and help decipher metabolic cancer pathways. We present here a novel droplet microfluidic method that allows the fast and quantitative determination of lactate release in many single cells. Using passive forces, droplets encapsulated cells are positioned in an array. The single-cell lactate release rate is determined from the increase in droplet fluorescence as the lactate is enzymatically converted to a fluorescent product. The method is used to measure the cell-to-cell variance of lactate release in K562 leukemia and U87 glioblastoma cancer cell lines and under the chemical inhibition of lactate efflux. The technique can be used in the study of cancer biology, but more broadly in cell biology, to capture the full range of stochastic variations in glycolysis activity in heterogeneous cell populations in a repeatable and high-throughput manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.