Evidence map›Paper›PMID 26900453›Full record

ArticleRSC advances

FACS-style detection for real-time cell viscoelastic cytometry.

A Kasukurti, C D Eggleton, S A Desai, D W M Marr

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

A KasukurtiDepartment of Chemical and Biological Engineering, Colorado School of Mines.
C D EggletonDepartment of Mechanical Engineering, University of Maryland, Baltimore County.
S A DesaiLaboratory for Malaria and Vector Research, National Institute of Allergy and Infectious Disease, Bethesda, MD.
D W M MarrDepartment of Chemical and Biological Engineering, Colorado School of Mines.

Funding

The Plasmodial Surface Anion Channel And Malaria Parasite Nutrient AcquisitionZIAAI000882 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI DESAI, SANJAY A · 2009 to 2025
$20.5M
High-Throughput Cell Mechanical Property Testing for Label-Free AssayingR01AI079347 · NIAID · COLORADO SCHOOL OF MINES · PI EGGLETON, CHARLES DIONISIO, MARR, DAVID WM · 2009 to 2012
$1.3M
NIAID NIH HHS R01 AI079347
6 · The paper itself

Abstract

Cell mechanical properties have been established as a label-free biophysical marker of cell viability and health; however, real-time methods with significant throughput for accurately and non-destructively measuring these properties remain widely unavailable. Without appropriate labels for use with fluorescence activated cell sorters (FACS), easily implemented real-time technology for tracking cell-level mechanical properties remains a current need. Employing modulated optical forces and enabled by a low-dimensional FACS-style detection method introduced here, we present a viscoelasticity cytometer (VC) capable of real-time and continuous measurements. We demonstrate the utility of this approach by tracking the high-frequency cell physical properties of populations of chemically-modified cells at rates of ~ 1 s

Identifiers

PMID26900453
PMCPMC4756765

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.