ArticleMolecular vision2016
Scleral fibroblast response to experimental glaucoma in mice.
Article in Molecular vision, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
35 citing papers in PubMed, 44 citations in OpenAlex.
- The Strain Response to Intraocular Pressure Decrease in the Lamina Cribrosa of Patients with Glaucoma.Ophthalmology. GlaucomaTrial
- Sparse Cell Labeling Reveals Morphologic Diversity Among Mouse Scleral Fibroblasts.Investigative ophthalmology & visual science · 2026Article
- Revisiting the sclera as a target for glaucoma therapy.Eye (London, England) · 2026Review
- The Effect of Prostaglandin F2 Analog Treatment on the Immunoexpression of Fibrosis-Associated Factors in Patients with Glaucoma Undergoing Deep Sclerotomy.International journal of molecular sciences · 2024Article
- The Mechanisms of Neuroprotection by Topical Rho Kinase Inhibition in Experimental Mouse Glaucoma and Optic Neuropathy.Investigative ophthalmology & visual science · 2024Article
- The relationship between intraocular pressure and glaucoma: An evolving concept.Progress in retinal and eye research · 2024Review
- IOP and glaucoma damage: The essential role of optic nerve head and retinal mechanosensors.Progress in retinal and eye research · 2024Review
- Comparison of the Biomechanics of the Mouse Astrocytic Lamina Cribrosa Between Glaucoma and Optic Nerve Crush Models.Investigative ophthalmology & visual science · 2023Article
- Scleral remodeling in early adulthood: the role of FGF-2.Scientific reports · 2023Article
- Angiotensin II-Related Activation of Scleral Fibroblasts and Their Role on Retinal Ganglion Cell Death in Glaucoma.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Chitosan-coated bovine serum albumin nanoparticles for topical tetrandrine delivery in glaucoma:Drug delivery · 2022Article
- Quantitative Microstructural Analysis of Cellular and Tissue Remodeling in Human Glaucoma Optic Nerve Head.Investigative ophthalmology & visual science · 2022Article
- Remodeling of the Lamina Cribrosa: Mechanisms and Potential Therapeutic Approaches for Glaucoma.International journal of molecular sciences · 2022Review
- Proteome alterations in the aqueous humor reflect structural and functional phenotypes in patients with advanced normal-tension glaucoma.Scientific reports · 2022Article
- Multiplex protein analysis for the study of glaucoma.Expert review of proteomics · 2021Review
- Scleraxis expressing scleral cells respond to inflammatory stimulation.Histochemistry and cell biology · 2021Article
- Changes in ocular morphology after cataract surgery in open angle glaucoma patients.Scientific reports · 2021Article
- Regional Differences and Physiologic Behaviors in Peripapillary Scleral Fibroblasts.Investigative ophthalmology & visual science · 2021Article
- Identification of key genes and pathways in scleral extracellular matrix remodeling in glaucoma: Potential therapeutic agents discovered using bioinformatics analysis.International journal of medical sciences · 2021Article
- Comparative proteome analysis of form-deprivation myopia in sclera with iTRAQ-based quantitative proteomics.Molecular vision · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo study the detailed cellular and molecular changes in the mouse sclera subjected to experimental glaucoma.
methodsThree strains of mice underwent experimental bead-injection glaucoma and were euthanized at 3 days and 1, 3, and 6 weeks. Scleral protein expression was analyzed with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) using (16)O/(18)O labeling for quantification in 1- and 6-week tissues. Sclera protein samples were also analyzed with immunoblotting with specific antibodies to selected proteins. The proportion of proliferating scleral fibroblasts was quantified with Ki67 and 4',6-diamidino-2-phenylindole (DAPI) labeling, and selected proteins were studied with immunohistochemistry.
resultsProteomic analysis showed increases in molecules involved in integrin-linked kinase signaling and actin cytoskeleton signaling pathways at 1 and 6 weeks after experimental glaucoma. The peripapillary scleral region had more fibroblasts than equatorial sclera (p=0.001, n=217, multivariable regression models). There was a sixfold increase in proliferating fibroblasts in the experimental glaucoma sclera at 1 week and a threefold rise at 3 and 6 weeks (p=0.0005, univariate regression). Immunoblots confirmed increases for myosin, spectrin, and actinin at 1 week after glaucoma. Thrombospondin-1 (TSP-1), HINT1, vimentin, actinin, and α-smooth muscle actin were increased according to immunohistochemistry.
conclusionsScleral fibroblasts in experimental mouse glaucoma show increases in actin cytoskeleton and integrin-related signaling, increases in cell division, and features compatible with myofibroblast transition.
Indexed as
Identifiers
26900327PMC4734151W2271759093What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.