ArticleJournal of assisted reproduction and genetics2016
Molecular analysis of DNA in blastocoele fluid using next-generation sequencing.
Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Diagnostic accuracy of initial serum β-hCG in predicting pregnancy outcomes post-SET in IVF/ICSI cycles: a systematic review and meta-analysis.Frontiers in endocrinology · 2026Pooled it
- Fragmentomic features of cell-free DNA in human spent blastocyst medium and their potential for non-invasive embryo ploidy assessment.Journal of assisted reproduction and genetics · 2026Article
- Comparative study of noninvasive chromosomal screening using blastocyst culture media: frozen-thawed embryos outperform fresh embryos.Frontiers in endocrinology · 2026Article
- Review
- Genomic aspects in reproductive medicine.Clinical and experimental reproductive medicine · 2024Article
- Evolution of Minimally Invasive and Non-Invasive Preimplantation Genetic Testing: An Overview.Journal of clinical medicine · 2024Review
- Comparing the advantages, disadvantages and diagnostic power of different non-invasive pre-implantation genetic testing: A literature review.International journal of reproductive biomedicine · 2023Review
- An Update on Non-invasive Approaches for Genetic Testing of the Preimplantation Embryo.Current genomics · 2022Review
- Apoptotic qPCR gene expression array analysis demonstrates proof-of-concept for rapid blastocoel fluid-conditioned media molecular prediction.Journal of assisted reproduction and genetics · 2022Article
- Validation of Non-Invasive Preimplantation Genetic Screening Using a Routine IVF Laboratory Workflow.Biomedicines · 2022Article
- Trophectoderm Biopsy Differentially Influences the Level of Serum β-Human Chorionic Gonadotropin With Different Embryonic Trophectoderm Scores in Early Pregnancy From 7847 Single-Blastocyst Transfer Cycles.Frontiers in endocrinology · 2022Article
- Ploidy Testing of Blastocoel Fluid for Screening May Be Technically Challenging and More Invasive Than That of Spent Cell Culture Media.Frontiers in physiology · 2022Article
- Karyotype of the Blastocoel Fluid Derived by Laser-Assisted Hatching Demonstrates a Low Agreement With the Trophectoderm.Frontiers in physiology · 2022Article
- Preimplantation Genetic Testing for Monogenic Conditions: Is Cell-Free DNA Testing the Next Step?Molecular diagnosis & therapy · 2021Review
- Electrospray mass spectrometry analysis of blastocoel fluid as a potential tool for bovine embryo selection.Journal of assisted reproduction and genetics · 2021Article
- Cell-free DNA discoveries in human reproductive medicine: providing a new tool for biomarker and genetic assays in ART.Journal of assisted reproduction and genetics · 2021Review
- Pre-implantation genetic testing: Past, present, future.Reproductive medicine and biology · 2021Review
- Minimally Invasive Cell-Free Human Embryo Aneuploidy Testing (miPGT-A) Utilizing Combined Spent Embryo Culture Medium and Blastocoel Fluid -Towards Development of a Clinical Assay.Scientific reports · 2020Article
- The status of preimplantation genetic testing in the UK and USA.Human reproduction (Oxford, England) · 2020Article
- A prospective study of non-invasive preimplantation genetic testing for aneuploidies (NiPGT-A) using next-generation sequencing (NGS) on spent culture media (SCM).Journal of assisted reproduction and genetics · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPreimplantation genetic testing (PGT) requires an invasive biopsy to obtain embryonic material for genetic analysis. The availability of a less invasive procedure would increase the overall efficacy of PGT. The aim of the study was to explore the potential of blastocoele fluid (BF) as an alternative source of embryonic DNA for PGT.
methodsCollection of BF was performed by aspiration with a fine needle prior to vitrification. BF DNA was subjected to whole-genome amplification (WGA) and analyzed by high-resolution next-generation sequencing (NGS).
resultsA high-quality WGA product was obtained from 8 of 11 (72.7 %) samples. Comparison of matching BF and blastomere samples showed that the genomic representation of sequencing reads was consistently similar with respect to density and regional coverage across the 24 chromosomes. A genome-wide survey of the sample sequencing data also indicated that BF was highly representative of known single gene sequences, and this observation was validated by PCR analyses of ten randomly selected genes, with an overall efficiency of 84 %.
conclusionThis study provides further evidence that BF is a promising alternative source of DNA for PGT.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.