ArticleScientific reports2016
Particle alignment reliability in single particle electron cryomicroscopy: a general approach.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- Emerging Themes in CryoEM─Single Particle Analysis Image Processing.Chemical reviews · 2022Review
- On bias, variance, overfitting, gold standard and consensus in single-particle analysis by cryo-electron microscopy.Acta crystallographica. Section D, Structural biology · 2022Article
- Advances in Xmipp for Cryo-Electron Microscopy: From Xmipp to Scipion.Molecules (Basel, Switzerland) · 2021Article
- Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Validation tests for cryo-EM maps using an independent particle set.Journal of structural biology: X · 2020Article
- Scipion web tools: Easy to use cryo-EM image processing over the web.Protein science : a publication of the Protein Society · 2018Article
- Guidelines for using Bsoft for high resolution reconstruction and validation of biomolecular structures from electron micrographs.Protein science : a publication of the Protein Society · 2018Article
- Quantitative analysis of 3D alignment quality: its impact on soft-validation, particle pruning and homogeneity analysis.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electron Microscopy is reaching new capabilities thanks to the combined effect of new technologies and new image processing methods. However, the reconstruction process is still complex, requiring many steps and elaborated optimization procedures. Therefore, the possibility to reach a wrong structure exists, justifying the need of robust statistical tests. In this work, we present a conceptually simple alignment test, which does not require tilt-pair images, to evaluate the alignment consistency between a set of projection images with respect to a given 3D density map. We test the approach on a number of problems in 3DEM, especially the ranking and evaluation of initial 3D volumes and high resolution 3D maps, where we show its usefulness in providing an objective evaluation for maps that have recently been subject to a strong controversy in the field. Additionally, this alignment statistical test can be linked to the early stages of structure solving of new complexes, streamlining the whole process.
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Registered trials
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