ArticleMatrix biology : journal of the International Society for Matrix Biology
The ameloblastin extracellular matrix molecule enhances bone fracture resistance and promotes rapid bone fracture healing.
Article in Matrix biology : journal of the International Society for Matrix Biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Microenvironment-mediated stem cell fate in periodontal tissue remodeling and repair.Bioactive materials · 2026Review
- Oligomerization Function of the Native Exon 5 Sequence of Ameloblastin Fused with Calmodulin.ACS omega · 2025Article
- Ameloblastin and its multifunctionality in amelogenesis: A review.Matrix biology : journal of the International Society for Matrix Biology · 2024Review
- Histological evaluation of the effects of bone morphogenetic protein 9 and angiopoietin 1 on bone healing.Journal of Taibah University Medical Sciences · 2023Article
- Revisiting ameloblastin; addressing the EMT-ECM axis above and beyond oral biology.Frontiers in cell and developmental biology · 2023Article
- The role of proteoglycan form of DMP1 in cranial repair.BMC molecular and cell biology · 2022Article
- Small extracellular vesicles from dental follicle stem cells provide biochemical cues for periodontal tissue regeneration.Stem cell research & therapy · 2022Article
- Targeted activation of androgen receptor signaling in the periosteum improves bone fracture repair.Cell death & disease · 2022Article
- Ischemia Injury: A New Method Accelerates Bone Healing in a Rat Tibia Fracture Model.BioMed research international · 2019Article
- Bone physiology as inspiration for tissue regenerative therapies.Biomaterials · 2018Review
- Whole transcriptome analysis identifies differentially regulated networks between osteosarcoma and normal bone samples.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- Ameloblastin Peptides Modulates the Osteogenic Capacity of Human Mesenchymal Stem Cells.Frontiers in physiology · 2017Article
- Phosphorylation Modulates Ameloblastin Self-assembly and CaFrontiers in physiology · 2017Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
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Abstract
The extracellular matrix (ECM) provides structural support, cell migration anchorage, cell differentiation cues, and fine-tuned cell proliferation signals during all stages of bone fracture healing, including cartilaginous callus formation, callus remodeling, and bony bridging of the fracture gap. In the present study we have defined the role of the extracellular matrix protein ameloblastin (AMBN) in fracture resistance and fracture healing of mouse long bones. To this end, long bones from WT and AMBN(Δ5-6) truncation model mice were subjected to biomechanical analysis, fracture healing assays, and stem cell colony formation comparisons. The effect of exogenous AMBN addition to fracture sites was also determined. Our data indicate that lack of a functional AMBN in the bone matrix resulted in 31% decreased femur bone mass and 40% reduced energy to failure. On a cellular level, AMBN function inhibition diminished the proliferative capacity of fracture repair callus cells, as evidenced by a 58% reduction in PCNA and a 40% reduction in Cyclin D1 gene expression, as well as PCNA immunohistochemistry. In terms of fracture healing, AMBN truncation was associated with an enhanced and prolonged chondrogenic phase, resulting in delayed mineralized tissue gene expression and delayed ossification of the fracture repair callus. Underscoring a role of AMBN in fracture healing, there was a 6.9-fold increase in AMBN expression at the fracture site one week after fracture, and distinct AMBN immunolabeling in the fracture gap. Finally, application of exogenous AMBN protein to bone fracture sites accelerated callus formation and bone fracture healing (33% increase in bone volume and 19% increase in bone mineral density), validating the findings of our AMBN loss of function studies. Together, these data demonstrate the functional importance of the AMBN extracellular matrix protein in bone fracture prevention and rapid fracture healing.
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