ArticleProbiotics and antimicrobial proteins2016
Identification of Bacillus Probiotics Isolated from Soil Rhizosphere Using 16S rRNA, recA, rpoB Gene Sequencing and RAPD-PCR.
Article in Probiotics and antimicrobial proteins, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 74 citations in OpenAlex.
- Volatile organic compounds from Bacillus sp. PAR690 Promote plant growth by regulating photosynthesis and nitrogen metabolism.BMC plant biology · 2026Article
- Whole-genome sequencing and comparative genomic analysis of a novel Bacillus subtilis strain YE16 isolated from yak dung.Scientific reports · 2026Article
- Symbiotic Enterococcus faecalis potentiates viral pathogenesis via fructose-1,6-bisphosphate-mediated insect gut epithelial damage.NPJ biofilms and microbiomes · 2025Article
- Inoculation of wheat seeds with spore-forming bacteria as a novel approach to enhance growth under climate change-induced stress conditions.Scientific reports · 2025Article
- Genetic Characterization and Symbiotic Performance of Soybean Rhizobia Under Cold and Water-Deficient Conditions in Poland.Plants (Basel, Switzerland) · 2025Article
- Review
- Postbiotic metabolites derived from lactobacillus fermentum as potent antiproliferative bioresources on HeLa cells with promising biocompatibility.BMC complementary medicine and therapies · 2024Article
- Isolation ofMicroorganisms · 2024Article
- Probio-Ichnos: A Database of Microorganisms with In Vitro Probiotic Properties.Microorganisms · 2024Article
- The isolation and identification of Bacillus velezensis ZN-S10 from vanilla (V. planifolia), and the microbial distribution after the curing process.Scientific reports · 2024Article
- Exploring Andean High-Altitude Lake Extremophiles through Advanced Proteotyping.Journal of proteome research · 2024Article
- Enhanced specificity of Bacillus metataxonomics using a tuf-targeted amplicon sequencing approach.ISME communications · 2023Article
- The Genetic and Phenotypic Diversity ofMarine drugs · 2023Article
- Evaluation of efficacy and mechanism ofFrontiers in microbiology · 2023Article
- Screening of Spore-Forming Bacteria with Probiotic Potential in Pristine Algerian Caves.Microbiology spectrum · 2022Article
- Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae).Scientific reports · 2022Article
- Isolation and Characterization of Phosphate Solubilizing Bacteria from Paddy Field Soils in Japan.Microbes and environments · 2022Article
- Article
- Valorization of agro-wastes for the biosynthesis and characterization of polyhydroxybutyrate by3 Biotech · 2021Article
- Molecular typing tools for identifying and characterizing lactic acid bacteria: a review.Food science and biotechnology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Some Bacillus species, especially Bacillus subtilis and Bacillus pumilus groups, have highly similar 16S rRNA gene sequences, which are hard to identify based on 16S rDNA sequence analysis. To conquer this drawback, rpoB, recA sequence analysis along with randomly amplified polymorphic (RAPD) fingerprinting was examined as an alternative method for differentiating Bacillus species. The 16S rRNA, rpoB and recA genes were amplified via a polymerase chain reaction using their specific primers. The resulted PCR amplicons were sequenced, and phylogenetic analysis was employed by MEGA 6 software. Identification based on 16S rRNA gene sequencing was underpinned by rpoB and recA gene sequencing as well as RAPD-PCR technique. Subsequently, concatenation and phylogenetic analysis showed that extent of diversity and similarity were better obtained by rpoB and recA primers, which are also reinforced by RAPD-PCR methods. However, in one case, these approaches failed to identify one isolate, which in combination with the phenotypical method offsets this issue. Overall, RAPD fingerprinting, rpoB and recA along with concatenated genes sequence analysis discriminated closely related Bacillus species, which highlights the significance of the multigenic method in more precisely distinguishing Bacillus strains. This research emphasizes the benefit of RAPD fingerprinting, rpoB and recA sequence analysis superior to 16S rRNA gene sequence analysis for suitable and effective identification of Bacillus species as recommended for probiotic products.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.