ArticleMolecular and cellular biology1989
The Saccharomyces cerevisiae PUT3 activator protein associates with proline-specific upstream activation sequences.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 75 citations in OpenAlex.
- Polygraph: a software framework for the systematic assessment of synthetic regulatory DNA elements.Genome biology · 2025Article
- Proline catabolism is a key factor facilitating Candida albicans pathogenicity.PLoS pathogens · 2023Article
- Yeast homologs of human MCUR1 regulate mitochondrial proline metabolism.Nature communications · 2020Article
- Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation.Developmental cell · 2020Article
- Mitochondrial proline catabolism activates Ras1/cAMP/PKA-induced filamentation in Candida albicans.PLoS genetics · 2019Article
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- Systematic Analysis of Transcriptional and Post-transcriptional Regulation of Metabolism in Yeast.PLoS computational biology · 2017Article
- ChiNet uncovers rewired transcription subnetworks in tolerant yeast for advanced biofuels conversion.Nucleic acids research · 2015Article
- Nuclear GPS for interchromosomal clustering.Developmental cell · 2012Article
- Transcription factor binding to a DNA zip code controls interchromosomal clustering at the nuclear periphery.Developmental cell · 2012Article
- Regulation of amino acid, nucleotide, and phosphate metabolism in Saccharomyces cerevisiae.Genetics · 2012Article
- Pushing the limits of what is achievable in protein-DNA docking: benchmarking HADDOCK's performance.Nucleic acids research · 2010Article
- MlcR, a zinc cluster activator protein, is able to bind to a single (A/T)CGG site of cognate asymmetric motifs in the ML-236B (compactin) biosynthetic gene cluster.Molecular genetics and genomics : MGG · 2009Article
- Article
- Genomic analysis of the hierarchical structure of regulatory networks.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- A fungal family of transcriptional regulators: the zinc cluster proteins.Microbiology and molecular biology reviews : MMBR · 2006Review
- Modulation of transcription factor function by an amino acid: activation of Put3p by proline.The EMBO journal · 2003Article
- Rapamycin treatment results in GATA factor-independent hyperphosphorylation of the proline utilization pathway activator in Saccharomyces cerevisiae.Eukaryotic cell · 2003Article
- Phenotypic analysis of genes encoding yeast zinc cluster proteins.Nucleic acids research · 2001Article
- The regulator of the yeast proline utilization pathway is differentially phosphorylated in response to the quality of the nitrogen source.Molecular and cellular biology · 2000Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
The PUT1 and PUT2 genes encoding the enzymes of the proline utilization pathway of Saccharomyces cerevisiae are induced by proline and activated by the product of the PUT3 gene. Two upstream activation sequences (UASs) in the PUT1 promoter were identified by homology to the PUT2 UAS. Deletion analysis of the two PUT1 UASs showed that they were functionally independent and additive in producing maximal levels of gene expression. The consensus PUT UAS is a 21-base-pair partially palindromic sequence required in vivo for induction of both genes. The results of a gel mobility shift assay demonstrated that the proline-specific UAS is the binding site of a protein factor. In vitro complex formation was observed in crude extracts of yeast strains carrying either a single genomic copy of the PUT3 gene or the cloned PUT3 gene on a 2 microns plasmid, and the binding was dosage dependent. DNA-binding activity was not observed in extracts of strains carrying either a put3 mutation that caused a noninducible (Put-) phenotype or a deletion of the gene. Wild-type levels of complex formation were observed in an extract of a strain carrying an allele of PUT3 that resulted in a constitutive (Put+) phenotype. Extracts from a strain carrying a PUT3-lacZ gene fusion formed two complexes of slower mobility than the wild-type complex. We conclude that the PUT3 product is either a DNA-binding protein or part of a DNA-binding complex that recognizes the UASs of both PUT1 and PUT2. Binding was observed in extracts of a strain grown in the presence or absence of proline, demonstrating the constitutive nature of the DNA-protein interaction.
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