ArticleMolecular and cellular biology1989
The HML mating-type cassette of Saccharomyces cerevisiae is regulated by two separate but functionally equivalent silencers.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 98 citations in OpenAlex.
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Evolution and Functional Trajectory of Sir1 in Gene Silencing.Molecular and cellular biology · 2016Article
- Nucleosome-positioning sequence repeats impact chromatin silencing in yeast minichromosomes.Genetics · 2014Article
- Review
- Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution.Science (New York, N.Y.) · 2011Article
- Article
- Design of a minimal silencer for the silent mating-type locus HML of Saccharomyces cerevisiae.Nucleic acids research · 2010Article
- Saccharomyces cerevisiae Esc2p interacts with Sir2p through a small ubiquitin-like modifier (SUMO)-binding motif and regulates transcriptionally silent chromatin in a locus-dependent manner.The Journal of biological chemistry · 2010Article
- Elaboration, diversification and regulation of the Sir1 family of silencing proteins in Saccharomyces.Genetics · 2009Article
- ABF1-binding sites promote efficient global genome nucleotide excision repair.The Journal of biological chemistry · 2009Article
- The DNA end-binding protein Ku regulates silencing at the internal HML and HMR loci in Saccharomyces cerevisiae.Genetics · 2008Article
- Positive roles of SAS2 in DNA replication and transcriptional silencing in yeast.Nucleic acids research · 2008Article
- Asymmetric positioning of nucleosomes and directional establishment of transcriptionally silent chromatin by Saccharomyces cerevisiae silencers.Molecular and cellular biology · 2006Article
- Position effect on the directionality of silencer function in Saccharomyces cerevisiae.Genetics · 2006Article
- Article
- Article
- REP3-mediated silencing in Saccharomyces cerevisiae.Genetics · 2004Article
- Article
- A methyltransferase targeting assay reveals silencer-telomere interactions in budding yeast.Molecular and cellular biology · 2003Article
- Domains of gene silencing near the left end of chromosome III in Saccharomyces cerevisiae.Genetics · 2002Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, most likely mediated through two cis-acting sites located on opposite sides of the locus. We showed that deletion of either of these two cis-acting sites from the chromosome did not yield any detectable derepression of HML, while deletion of both sites yielded full expression of the locus. In addition, each of these sites was capable of exerting repression of heterologous genes inserted in their vicinity. Thus, HML expression is regulated by two independent silencers, each fully competent for maintaining repression. This situation was distinct from the organization of the other silent locus, HMR, at which a single silencer served as the predominant repressor of expression. Examination of identifiable domains and binding sites within the HML silencers suggested that silencing activity can be achieved by a variety of combinations of various functional domains.
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