ReviewSystematic parasitology2016
Molecular approaches to trematode systematics: 'best practice' and implications for future study.
Review in Systematic parasitology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed.
- Three new species of Macvicaria Gibson & Bray, 1982 (Digenea: Opecoelidae) infecting Diplodus spp. (Teleostei, Sparidae) from South Africa and Namibia.Systematic parasitology · 2026Article
- Resurrection of Astiotrema varanum (Verma, 1930) Mukherjee & Ghosh, 1970 with Restriction of Astiotrema impletum (Looss, 1899) Looss, 1900 to Freshwater African Tetraodontid Records.Acta parasitologica · 2026Article
- Bridging knowledge gaps in two trematode life cycles: insights from southern African freshwater ecosystems.International journal for parasitology. Parasites and wildlife · 2026Review
- Reorganisation of the turtle blood fluke genera Hapalotrema and Learedius (Trematoda: Hapalotrematidae), with the proposal of two new genera, a new species, and comments on population structure.Systematic parasitology · 2026Article
- Balanocochlis glans (Gastropoda: Thiaridae) as a New Intermediate Host of Centrocestus formosanus (Digenea: Heterophyidae) in the Philippines.Acta parasitologica · 2026Article
- Integrating eDNA, molecular cercariometry, and snail surveys enhances characterization of digenetic trematode diversity.Parasitology research · 2026Article
- Ecological influences on host-parasite dynamics amongFrontiers in parasitology · 2026Article
- Emerging zoonotic risk: molecular detection ofScience in One Health · 2026Article
- Occurrence of brachylaimid trematodes, alive and shell-trapped, in sympatric species of terrestrial gastropods in France.Parasite (Paris, France) · 2026Article
- Assessing whole-host homogenisation as a new tool for parasite detection and identification.Current research in parasitology & vector-borne diseases · 2026Article
- Hidden in morphology, revealed by molecular genetics: synonymization ofParasitology · 2025Article
- Trematode Infection Prevalence Increases With Snail Richness: Observations From a 4-Year Study of Snail-Trematode Interactions.Ecology and evolution · 2025Article
- A new family for Cephalotrema elasticum (Digenea) based on molecular data, with notes on the status of the definitive host population.Scientific reports · 2025Article
- Postglacial Echoes: Parasite Genomics Uncover Environmental Changes in Postglacial European Lakes.Molecular ecology · 2025Article
- Back from the dead: validity and taxonomic position ofParasitology · 2025Article
- Phylogenetic and genetic evolutionary analyses of the mitochondrial genome ofMicrobiology spectrum · 2025Article
- Fecal metabarcoding vs traditional invasive survey to access the diversity of anuran endoparasites and diet.Parasitology · 2025Article
- Intermediate insights: tracing trematodes infecting amphibians via their first intermediate snail hosts.Parasites & vectors · 2025Article
- Systematic revision of the genus Orchidasma Looss, 1900 and description of Orchidasma orchilobata n. sp. from the loggerhead Caretta caretta (L.) and Kemp's ridley Lepidochelys kempii (Garman) turtles.Systematic parasitology · 2025Article
- First Report of Proctoeces maculatus (Digenea: Fellodistomidae) Infecting the Ribbed Mussel, Geukensia demissa: Detection of a Unique Haplotype in New England, USA.Acta parasitologica · 2025Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, morphological analysis has been the cornerstone to trematode systematics. However, since the late-1980s we have seen an increased integration of genetic data to overcome problems encountered when morphological data are considered in isolation. Here, we provide advice regarding the 'best molecular practice' for trematode taxonomy and systematic studies, in an attempt to help unify the field and provide a solid foundation to underpin future work. Emphasis is placed on defining the study goals and recommendations are made regarding sample preservation, extraction methods, and the submission of molecular vouchers. We advocate generating sequence data from all parasite species/host species/geographic location combinations and stress the importance of selecting two independently evolving loci (one ribosomal and one mitochondrial marker). We recommend that loci should be chosen to provide genetic variation suitable to address the question at hand and for which sufficient 'useful' comparative sequence data already exist. Quality control of the molecular data via using proof-reading Taq polymerase, sequencing PCR amplicons using both forward and reverse primers, ensuring that a minimum of 85% overlap exists when constructing consensus sequences, and checking electropherograms by eye is stressed. We advise that all genetic results are best interpreted using a holistic biological approach, which considers morphology, host identity, collection locality, and ecology. Finally, we consider what advances next-generation sequencing holds for trematode taxonomy and systematics.
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