ArticleScientific reports2016
Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Genetically modified mice as a tool for the study of human diseases.Molecular biology reports · 2024Review
- Modeling sarcoma relevant translocations using CRISPR-Cas9 in human embryonic stem derived mesenchymal precursors.Genes, chromosomes & cancer · 2023Review
- A gap-free genome assembly of Chlamydomonas reinhardtii and detection of translocations induced by CRISPR-mediated mutagenesis.Plant communications · 2023Article
- CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops.Molecular biotechnology · 2022Review
- i-CRISPR: a personalized cancer therapy strategy through cutting cancer-specific mutations.Molecular cancer · 2022Article
- Universal toxin-based selection for precise genome engineering in human cells.Nature communications · 2021Article
- CRISPR Gene Editing of Murine Blood Stem and Progenitor Cells Induces MLL-AF9 Chromosomal Translocation and MLL-AF9 Leukaemogenesis.International journal of molecular sciences · 2020Article
- Variability in Genome Editing Outcomes: Challenges for Research Reproducibility and Clinical Safety.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Review
- Applications and explorations of CRISPR/Cas9 in CAR T-cell therapy.Briefings in functional genomics · 2020Review
- CRISPR-Cas systems: Overview, innovations and applications in human disease research and gene therapy.Computational and structural biotechnology journal · 2020Review
- Rare or Overlooked? Structural Disruption of Regulatory Domains in Human Neurocristopathies.Frontiers in genetics · 2020Review
- Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii.BMC biotechnology · 2019Article
- Tissue-restricted genome editing in vivo specified by microRNA-repressible anti-CRISPR proteins.RNA (New York, N.Y.) · 2019Article
- Induction of t(11;14) IgH enhancer/promoter-Oncology letters · 2019Article
- Article
- Stem cells and genome editing: approaches to tissue regeneration and regenerative medicine.Journal of human genetics · 2018Review
- Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing.Scientific reports · 2018Article
- One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR).PloS one · 2018Article
- Induction of Chromosomal Translocations with CRISPR-Cas9 and Other Nucleases: Understanding the Repair Mechanisms That Give Rise to Translocations.Advances in experimental medicine and biology · 2018Review
- Applications of Alternative Nucleases in the Age of CRISPR/Cas9.International journal of molecular sciences · 2017Review
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosomal translocation is the most common form of chromosomal abnormality and is often associated with congenital genetic disorders, infertility, and cancers. The lack of cellular and animal models for chromosomal translocations, however, has hampered our ability to understand the underlying disease mechanisms and to develop new therapies. Here, we show that site-specific chromosomal translocations can be generated in mouse embryonic stem cells (mESCs) via CRISPR/Cas9. Mouse ESCs carrying translocated chromosomes can be isolated and expanded to establish stable cell lines. Furthermore, chimeric mice can be generated by injecting these mESCs into host blastocysts. The establishment of ESC-based cellular and animal models of chromosomal translocation by CRISPR/Cas9 provides a powerful platform for understanding the effect of chromosomal translocation and for the development of new therapeutic strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.