Evidence map›Paper›PMID 26897546›Full record

ArticleJournal of neuroinflammation2016

Bilateral neuroinflammatory processes in visual pathways induced by unilateral ocular hypertension in the rat.

Anaïs Sapienza, Anne-Laure Raveu, Elodie Reboussin, Christophe Roubeix, Céline Boucher, Julie Dégardin, David Godefroy, William Rostène, Annabelle Reaux-Le Goazigo, Christophe Baudouin and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
3.0field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 69 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Review
  18. The heterogeneity of astrocytes in glaucoma.Frontiers in neuroanatomy · 2022
    Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Anaïs SapienzaSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
Anne-Laure RaveuSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
Elodie ReboussinSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
Christophe RoubeixSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
Céline BoucherSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
Julie DégardinSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
David GodefroySorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
William RostèneSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
Annabelle Reaux-Le GoazigoSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
Christophe BaudouinSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France.
Stéphane Melik ParsadaniantzSorbonne Universités, UPMC University of Paris 06, Institut de la Vision, 17 rue Moreau, 75012, Paris, France. stephane.melik-parsadaniantz@inserm.fr.
Centre National de la Recherche Scientifique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGlaucoma is one of the leading causes of irreversible blindness in the world. The major risk factor is elevated intraocular pressure (IOP) leading to progressive retinal ganglion cell (RGC) death from the optic nerve (ON) to visual pathways in the brain. Glaucoma has been reported to share mechanisms with neurodegenerative disorders. We therefore hypothesize that neuroinflammatory mechanisms in central visual pathways may contribute to the spread of glaucoma disease. The aim of the present study was to analyze the neuroinflammation processes that occur from the pathological retina to the superior colliculi (SCs) in a rat model of unilateral ocular hypertension induced by episcleral vein cauterization (EVC).

resultsSix weeks after unilateral (right eye) EVC in male Long-Evans rats, we evaluated both the neurodegenerative process and the neuroinflammatory state in visual pathway tissues. RGCs immunolabeled (Brn3a(+)) in ipsilateral whole flat-mounted retina demonstrated peripheral RGC loss associated with tissue macrophage/microglia activation (CD68(+)). Gene expression analysis of hypertensive and normotensive retinas revealed a significant increase of pro-inflammatory genes such as CCL2, IL-1β, and Nox2 mRNA expression compared to naïve eyes. Importantly, we found an upregulation of pro-inflammatory markers such as IL-1β and TNFα and astrocyte and tissue macrophage/microglia activation in hypertensive and normotensive RGC projection sites in the SCs compared to a naïve SC. To understand how neuroinflammation in the hypertensive retina is sufficient to damage both right and left SCs and the normotensive retina, we used an inflammatory model consisting in an unilateral stereotaxic injection of TNFα (25 ng/μl) in the right SC of naïve rats. Two weeks after TNFα injection, using an optomotor test, we observed that rats had visual deficiency in both eyes. Furthermore, both SCs showed an upregulation of genes and proteins for astrocytes, microglia, and pro-inflammatory cytokines, notably IL-1β. In addition, both retinas exhibited a significant increase of inflammatory markers compared to a naïve retina.

conclusionsAll these data evidence the complex role played by the SCs in the propagation of neuroinflammatory events induced by unilateral ocular hypertension and provide a new insight into the spread of neurodegenerative diseases such as glaucoma.

Indexed as

AnimalsAntigens, CDCalcium-Binding ProteinsCholera ToxinCytokinesDisease Models, AnimalEncephalitisFunctional LateralityGlial Fibrillary Acidic ProteinMaleMicrofilament ProteinsOcular HypertensionOptometryOrganic ChemicalsProto-Oncogene Proteins c-fosRatsAif1 protein, ratAlexa594Antigens, CDCalcium-Binding ProteinsCholera ToxinCytokinesGlial Fibrillary Acidic ProteinMicrofilament ProteinsOrganic ChemicalsProto-Oncogene Proteins c-fosTumor Necrosis Factor-alpha

Identifiers

PMID26897546
PMCPMC4761202
OpenAlexW2281405694

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.