ArticleBiosensors & bioelectronics2016
Label-free electrochemical impedance biosensor to detect human interleukin-8 in serum with sub-pg/ml sensitivity.
Article in Biosensors & bioelectronics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Recent advances in the biosensing platforms for sepsis diagnosis.RSC advances · 2026Review
- Advances in biosensors for diagnosis and monitoring of inflammatory bowel disease: A review.Bioengineering & translational medicine · 2025Review
- A Proof-of-Concept Study on Bioelectric-Based Biosensing for Prostate-Specific Antigen Detection in Serum Samples.Biosensors · 2025Article
- Evaluation of Non-Faradaic Impedimetric Parameters for IL-8 Detection Using Gold Interdigitated Electrode-Based Biosensors: Towards Early Detection of Newborn Disability.Micromachines · 2025Article
- Molecular Biomarkers for Timely and Personalized Prediction of Maternal-Fetal Health Risk.Biomolecules · 2025Review
- Acoustic therapy for allergic rhinitis and chronic rhinosinusitis: modulating microbiome, immunity and well-being.Frontiers in allergy · 2025Review
- Current Scenario in Associating Clinical COVID-19 Biomarkers for Developing Surveillance Platforms.Current medicinal chemistry · 2025Review
- Review
- A Simple Sandwich Electrochemical Immunosensor for Rapid Detection of the Alzheimer's Disease Biomarker Tau Protein.Biosensors · 2024Article
- Nano-modified screen-printed electrode-based electrochemical immunosensors for oral cancer biomarker detection in undiluted human serum and saliva samples.Nanoscale advances · 2024Article
- Wearing the Lab: Advances and Challenges in Skin-Interfaced Systems for Continuous Biochemical Sensing.Advances in biochemical engineering/biotechnology · 2024Review
- Chimeric Protein Switch Biosensors.Advances in biochemical engineering/biotechnology · 2024Review
- MoSDiagnostics (Basel, Switzerland) · 2023Article
- Highly Sensitive Readout Interface for Real-Time Differential Precision Measurements with Impedance Biosensors.Biosensors · 2023Article
- Thermodynamics and Kinetics of Electron Transfer of Electrode-Immobilized Small Laccase fromMolecules (Basel, Switzerland) · 2022Article
- Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures.Biomedicines · 2022Review
- Sensing Inflammation Biomarkers with Electrolyte-Gated Organic Electronic Transistors.Advanced healthcare materials · 2021Review
- Biosensing strategies for the electrochemical detection of viruses and viral diseases - A review.Analytica chimica acta · 2021Review
- Longitudinal Zeolite-Iron Oxide Nanocomposite Deposited Capacitance Biosensor for Interleukin-3 in Sepsis Detection.Nanoscale research letters · 2021Article
- Electrochemical Biosensors for Cytokine Profiling: Recent Advancements and Possibilities in the Near Future.Biosensors · 2021Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Biosensors with high sensitivity and short time-to-result that are capable of detecting biomarkers in body fluids such as serum are an important prerequisite for early diagnostics in modern healthcare provision. Here, we report the development of an electrochemical impedance-based sensor for the detection in serum of human interleukin-8 (IL-8), a pro-angiogenic chemokine implicated in a wide range of inflammatory diseases. The sensor employs a small and robust synthetic non-antibody capture protein based on a cystatin scaffold that displays high affinity for human IL-8 with a KD of 35 ± 10 nM and excellent ligand specificity. The change in the phase of the electrochemical impedance from the serum baseline, ∆θ(ƒ), measured at 0.1 Hz, was used as the measure for quantifying IL-8 concentration in the fluid. Optimal sensor signal was observed after 15 min incubation, and the sensor exhibited a linear response versus logarithm of IL-8 concentration from 900 fg/ml to 900 ng/ml. A detection limit of around 90 fg/ml, which is significantly lower than the basal clinical levels of 5-10 pg/ml, was observed. Our results are significant for the development of point-of-care and early diagnostics where high sensitivity and short time-to-results are essential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.