Evidence map›Paper›PMID 26896856›Full record

ArticleStem cell research2016

A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells.

Sheyla González, Hua Mei, Martin N Nakatsu, Elfren R Baclagon, Sophie X Deng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Article
  3. Concise Review: Bioengineering of Limbal Stem Cell Niche.Bioengineering (Basel, Switzerland) · 2023
    Review
  4. Review
  5. Article
  6. Regulation of Limbal Epithelial Stem Cells: Importance of the Niche.International journal of molecular sciences · 2021
    Review
  7. Review
  8. Limbal stem cell diseases.Experimental eye research · 2021
    Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sheyla GonzálezCornea Division, Stein Eye Institute, University of California, Los Angeles, CA, USA.
Hua MeiCornea Division, Stein Eye Institute, University of California, Los Angeles, CA, USA.
Martin N NakatsuCornea Division, Stein Eye Institute, University of California, Los Angeles, CA, USA.
Elfren R BaclagonCornea Division, Stein Eye Institute, University of California, Los Angeles, CA, USA.
Sophie X DengCornea Division, Stein Eye Institute, University of California, Los Angeles, CA, USA. Electronic address: deng@jsei.ucla.edu.

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Gregory Darin Field, DAVID S WILLIAMS · 1985 to 2026
$16.8M
Ex Vivo Expansion of Human Limbal Stem Cells for TransplantationR01EY021797 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Sophie Deng · 2012 to 2026
$5.1M
NEI NIH HHS 1R01EY021797NEI NIH HHS 5P30EY000331NEI NIH HHS P30 EY000331NEI NIH HHS R01 EY021797
6 · The paper itself

Abstract

The standard method of cultivating limbal epithelial progenitor/stem cells (LSCs) on a monolayer of mouse 3T3 feeder cells possesses the risk of cross-contamination in clinical applications. Human feeder cells have been used to eliminate this risk; however, efficiency from xenobiotic-free cultures on a monolayer appears to be lower than in the standard method using 3T3 cells. We investigated whether bone marrow stromal cells (BMSCs), also known as bone marrow-derived mesenchymal stem cells, could serve as feeder cells for the expansion of LSCs in the 3-dimensional (3D) system. Primary single human LSCs on a monolayer of 3T3s served as the control. Very poor growth was observed when single LSCs were cultured on BMSCs. When LSC clusters were cultured on a BMSC monolayer (CC-BM), 3D culture system (3D CC-BM) and fibrin 3D system (fibrin 3D CC-BM), the 3D CC-BM method supported a greater LSC expansion. The 3D CC-BM system produced a 2.5-fold higher cell growth rate than the control (p<0.05). The proportion of K14(+) and p63α(bright) cells was comparable to those in the control (p>0.05), whereas the proportion of K12(+) cells was lower (p<0.05). These results indicate that BMSCs can efficiently support the expansion of the LSC population in the 3D culture.

Indexed as

Cell Culture Techniques3T3 CellsAnimalsAntigens, CD34Bone Marrow CellsCadherinsCells, CulturedEpithelial CellsFeeder CellsHumansImmunohistochemistryLeukocyte Common AntigensLimbus CorneaeMesenchymal Stem CellsMiceMicroscopyAntigens, CD34CadherinsLeukocyte Common AntigensBone marrow derived-mesenchymal stem cellBone marrow stromal cellsCorneal epitheliumLimbal stem cell deficiencyLimbal stem cells

Identifiers

PMID26896856
PMCPMC4828302

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.