ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2016
Progressive cell-mediated changes in articular cartilage and bone in mice are initiated by a single session of controlled cyclic compressive loading.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Methodological aspects ofJournal of musculoskeletal & neuronal interactions · 2023Pooled it
- Trial
- Joint damage is more severe following a single bout than multiple bouts of high magnitude loading in mice.Osteoarthritis and cartilage · 2026Article
- Cathepsin K Inhibitors as Potential Drugs for the Treatment of Osteoarthritis.International journal of molecular sciences · 2025Review
- Animal Models of Osteoarthritis: Updated Models and Outcome Measures 2016-2023.Regenerative engineering and translational medicine · 2024Article
- Mechanical loading of joint modulates T cells in lymph nodes to regulate osteoarthritis.Osteoarthritis and cartilage · 2024Article
- Role and Mechanism of Mechanical Load in the Homeostasis of the Subchondral Bone in Knee Osteoarthritis: A Comprehensive Review.Journal of inflammation research · 2024Review
- Posttraumatic osteoarthritis: from basic science to clinical implications.OTA international : the open access journal of orthopaedic trauma · 2023Article
- Review
- Small-Animal Compression Models of Osteoarthritis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Contribution of joint tissue properties to load-induced osteoarthritis.Bone reports · 2022Article
- Article
- Article
- Early inhibition of subchondral bone remodeling slows load-induced posttraumatic osteoarthritis development in mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2021Article
- A Non-Invasive Method for Generating the Cyclic Loading-Induced Intra-Articular Cartilage Lesion Model of the Rat Knee.Journal of visualized experiments : JoVE · 2021Article
- Obesity and load-induced posttraumatic osteoarthritis in the absence of fracture or surgical trauma.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Pathogenesis and clinical management of obesity-related knee osteoarthritis: Impact of mechanical loading.Journal of orthopaedic translation · 2020Review
- Injectable mechanical pillows for attenuation of load-induced post-traumatic osteoarthritis.Regenerative biomaterials · 2019Article
- Mechanobiological Mechanisms of Load-Induced Osteoarthritis in the Mouse Knee.Journal of biomechanical engineering · 2019Article
- Ablation of low-molecular-weight FGF2 isoform accelerates murine osteoarthritis while loss of high-molecular-weight FGF2 isoforms offers protection.Journal of cellular physiology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
We previously showed that repetitive cyclic loading of the mouse knee joint causes changes that recapitulate the features of osteoarthritis (OA) in humans. By applying a single loading session, we characterized the temporal progression of the structural and compositional changes in subchondral bone and articular cartilage. We applied loading during a single 5-minute session to the left tibia of adult (26-week-old) C57Bl/6 male mice at a peak load of 9.0N for 1,200 cycles. Knee joints were collected at times 0, 1, and 2 weeks after loading. The changes in articular cartilage and subchondral bone were analyzed by histology, immunohistochemistry (caspase-3 and cathepsin K), and microcomputed tomography. At time 0, no change was evident in chondrocyte viability or cartilage or subchondral bone integrity. However, cartilage pathology demonstrated by localized thinning and proteoglycan loss occurred at 1 and 2 weeks after the single session of loading. Transient cancellous bone loss was evident at 1 week, associated with increased osteoclast number. Bone loss was reversed to control levels at 2 weeks. We observed formation of fibrous and cartilaginous tissues at the joint margins at 1 and 2 weeks. Our findings demonstrate that a single session of noninvasive loading leads to the development of OA-like morphological and cellular alterations in articular cartilage and subchondral bone. The loss in subchondral trabecular bone mass and thickness returns to control levels at 2 weeks, whereas the cartilage thinning and proteoglycan loss persist. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1941-1949, 2016.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.