ArticleeLife2016
A genome-wide resource for the analysis of protein localisation in Drosophila.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 223 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
223 citing papers in PubMed, 403 citations in OpenAlex.
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- Mmp2 regulates basement membrane remodeling and dedifferentiation of the visceral musculature during Drosophila metamorphosis.Scientific reports · 2026Article
- Octopamine signaling from clock neurons plays dual roles in Drosophila long-term memory.PLoS genetics · 2026Article
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- DynamicAtlas: a morphodynamic atlas for Drosophila development.Nature methods · 2026Article
- Analysis of Muscle Structure Using Fluorescently Tagged Proteins in Drosophila melanogaster Femur Muscles.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Targeting and anchoring the mechanosensitive ion channel Piezo to facilitate its inhibition of axon regeneration.PLoS genetics · 2025Article
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163 more citing papers are in PubMed but not listed here.
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Authors and funding
26 authors at 6 institutions in 4 countries.
Funding
Abstract
The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.