ArticleG3 (Bethesda, Md.)2016
Embryonal Control of Yellow Seed Coat Locus ECY1 Is Related to Alanine and Phenylalanine Metabolism in the Seed Embryo of Brassica napus.
Article in G3 (Bethesda, Md.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 35 citations in OpenAlex.
- A rare dominant alleleScience advances · 2025Article
- Current Insights into Weak Seed Dormancy and Pre-Harvest Sprouting in Crop Species.Plants (Basel, Switzerland) · 2024Review
- Identification of Yellow Seed Color Genes Using Bulked Segregant RNA Sequencing inInternational journal of molecular sciences · 2024Article
- De novo transcriptome assembly of Dalbergia sissoo Roxb. (Fabaceae) under Botryodiplodia theobromae-induced dieback disease.Scientific reports · 2023Article
- Interaction between phenylpropane metabolism and oil accumulation in the developing seed of Brassica napus revealed by high temporal-resolution transcriptomes.BMC biology · 2023Article
- Comparative genomic analyses reveal the genetic basis of the yellow-seed trait in Brassica napus.Nature communications · 2023Article
- QTL Mapping and Transcriptome Analysis Reveal Candidate Genes Regulating Seed Color inInternational journal of molecular sciences · 2023Article
- A novel TF molecular switch-mechanism found in two contrasting ecotypes of a psammophyte, Agriophyllum squarrosum, in regulating transcriptional drought memory.BMC plant biology · 2023Article
- Exploring the Mystery of the Tetrahydrobiopterin Synthetic Defect Lethal MutantInternational journal of molecular sciences · 2022Article
- Multi-omics analysis dissects the genetic architecture of seed coat content in Brassica napus.Genome biology · 2022Article
- Genetic Mapping and Identification of the Candidate Gene for White Seed Coat inInternational journal of molecular sciences · 2021Article
- Article
- Grain dormancy genes responsible for preventing pre-harvest sprouting in barley and wheat.Breeding science · 2018Review
- Alanine aminotransferase controls seed dormancy in barley.Nature communications · 2016Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seed coat color is determined by the type of pigment deposited in the seed coat cells. It is related to important agronomic traits of seeds such as seed dormancy, longevity, oil content, protein content and fiber content. In Brassica napus, inheritance of seed coat color is related to maternal effects and pollen effects (xenia effects). In this research we isolated a mutation of yellow seeded B. napus controlled by a single Mendelian locus, which is named Embryonal Control of Yellow seed coat 1 (Ecy1). Microscopy of transverse sections of the mature seed show that pigment is deposited only in the outer layer of the seed coat. Using Illumina Hisequation 2000 sequencing technology, a total of 12 GB clean data, 116× coverage of coding sequences of B. napus, was achieved from seeds 26 d after pollination (DAP). It was assembled into 172,238 independent transcripts, and 55,637 unigenes. A total of 139 orthologous genes of Arabidopsis transparent testa (TT) genes were mapped in silico to 19 chromosomes of B. napus Only 49 of the TT orthologous genes are transcribed in seeds. However transcription of all orthologs was independent of embryonal control of seed coat color. Only 55 genes were found to be differentially expressed between brown seeds and the yellow mutant. Of these 55, 50 were upregulated and five were downregulated in yellow seeds as compared to their brown counterparts. By KEGG classification, 14 metabolic pathways were significantly enriched. Of these, five pathways: phenylpropanoid biosynthesis, cyanoamino acid metabolism, plant hormone signal transduction, metabolic pathways, and biosynthesis of secondary metabolites, were related with seed coat pigmentation. Free amino acid quantification showed that Ala and Phe were present at higher levels in the embryos of yellow seeds as compared to those of brown seeds. This increase was not observed in the seed coat. Moreover, the excess amount of free Ala was exactly twice that of Phe in the embryo. The pigment substrate chalcone is synthesized from two molecules of Ala and one molecule of Phe. The correlation between accumulation of Ala and Phe, and disappearance of pigment in the yellow seeded mutant, suggests that embryonal control of seed coat color is related with Phe and Ala metabolism in the embryo of B. napus.
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