ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2016
Immediate stabilization of human blood for delayed quantification of endogenous thiols and disulfides.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01265563 (Correction of Glutathione Deficiency for Treatment of Diabetic Nephropathy), which is not on this map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Correction of Glutathione Deficiency for Treatment of Diabetic Nephropathy
Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- An Artifact-Free Assay for the GSH/GSSG Ratio Adapted for Finger-Stick Blood Microvolumes: Simple, Sensitive, and Suitable for Any Laboratory.Antioxidants (Basel, Switzerland) · 2026Article
- Homocysteine: Canary in the Coal Mine or Hidden Threat? A Biochemical Study on the Role of Plasma Thiols.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Measurement of S-glutathionylated proteins by HPLC.Amino acids · 2022Article
- Blood Thiol Redox State in Chronic Kidney Disease.International journal of molecular sciences · 2022Review
- Blood Plasma Quality Control by Plasma Glutathione Status.Antioxidants (Basel, Switzerland) · 2021Article
- Common and Novel Markers for Measuring Inflammation and Oxidative Stress Ex Vivo in Research and Clinical Practice-Which to Use Regarding Disease Outcomes?Antioxidants (Basel, Switzerland) · 2021Review
- Protective Effect of Crocin against Mitochondrial Damage and Memory Deficit Induced by Beta-amyloid in the Hippocampus of Rats.Iranian journal of pharmaceutical research : IJPR · 2021Article
- Addressing Glutathione Redox Status in Clinical Samples by Two-Step Alkylation with N-ethylmaleimide Isotopologues.Metabolites · 2020Article
- Anethole Dithiolethione Increases Glutathione in Kidney by InhibitingOxidative medicine and cellular longevity · 2020Article
- Article
- A robust and versatile mass spectrometry platform for comprehensive assessment of the thiol redox metabolome.Redox biology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Endogenous thiols undergo rapid and reversible oxidation to disulfides when exposed to oxidants and are, therefore, suitable biomarkers of oxidative stress. However, accurate analysis of thiols in blood is frequently compromised by their artifactual oxidation during sample manipulation, which spuriously elevates the disulfide levels. Here, we describe a validated pre-analytical procedure that prevents both artifactual oxidation of thiols during sample manipulation and their oxidative decay for months in biosamples that are stored at -80°C. Addition of N-ethylmaleimide to blood samples from healthy donors was used to stabilize whole blood, red blood cells, platelets and plasma disulfides, whereas addition of citrate buffer followed by dilution of plasma with H2O was used to stabilize plasma thiols. The concentrations of thiols and disulfides were stable in all biosamples for at least 6 months when analyzed by UV/Vis HPLC at regular intervals. Only 3 ml of blood were needed to perform the analyses of thiols and disulfides in the different blood fractions. This pre-analytical procedure is reliable for use in both animal and human prospective studies. Its ease of implementation makes the method suitable for application to multicenter studies where blood samples are collected by different sites and personnel and are shipped to specific specialized laboratories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.