ArticleApplied and environmental microbiology2016
Engineering Escherichia coli for Microbial Production of Butanone.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 27 citations in OpenAlex.
- Synthetic metabolism forRSC chemical biology · 2022Article
- High-throughput colorimetric assays optimized for detection of ketones and aldehydes produced by microbial cell factories.Microbial biotechnology · 2022Article
- Microbial production of advanced biofuels.Nature reviews. Microbiology · 2021Review
- C4 Bacterial Volatiles Improve Plant Health.Pathogens (Basel, Switzerland) · 2021Review
- Feasible-metabolic-pathway-exploration technique using chemical latent space.Bioinformatics (Oxford, England) · 2020Article
- Metabolic engineering of β-oxidation to leverage thioesterases for production of 2-heptanone, 2-nonanone and 2-undecanone.Metabolic engineering · 2020Article
- Metabolic Engineering Design Strategies for Increasing Acetyl-CoA Flux.Metabolites · 2020Review
- Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol.Biotechnology for biofuels · 2020Article
- Short-chain ketone production by engineered polyketide synthases in Streptomyces albus.Nature communications · 2018Article
- Engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using unrelated carbon sources.Scientific reports · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To expand the chemical and molecular diversity of biotransformation using whole-cell biocatalysts, we genetically engineered a pathway in Escherichia coli for heterologous production of butanone, an important commodity ketone. First, a 1-propanol-producing E. coli host strain with its sleeping beauty mutase (Sbm) operon being activated was used to increase the pool of propionyl-coenzyme A (propionyl-CoA). Subsequently, molecular heterofusion of propionyl-CoA and acetyl-CoA was conducted to yield 3-ketovaleryl-CoA via a CoA-dependent elongation pathway. Lastly, 3-ketovaleryl-CoA was channeled into the clostridial acetone formation pathway for thioester hydrolysis and subsequent decarboxylation to form butanone. Biochemical, genetic, and metabolic factors affecting relative levels of ketogenesis, acidogenesis, and alcohol genesis under selected fermentative culture conditions were investigated. Using the engineered E. coli strain for batch cultivation with 30 g liter(-1)glycerol as the carbon source, we achieved coproduction of 1.3 g liter(-1)butanone and 2.9 g liter(-1)acetone. The results suggest that approximately 42% of spent glycerol was utilized for ketone biosynthesis, and thus they demonstrate potential industrial applicability of this microbial platform.
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