ArticleFEMS yeast research2016
Alternative reactions at the interface of glycolysis and citric acid cycle in Saccharomyces cerevisiae.
Article in FEMS yeast research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- Beyond Correlation: Constraint Architecture Explains Proteome-Metabolome Decoupling.International journal of molecular sciences · 2026Review
- Effects and function of citric acid on fermentation quality and microbial community in sugarcane tops silage with high and low water-soluble carbohydrate content.BMC plant biology · 2025Article
- Unlocking microbial interactions: Multi-Plant-based-substrate fermentation with water kefir starters for functional beverage innovation.Current research in food science · 2025Article
- Enhancing bioactive profiles of elderberry juice through yeast fermentation: a pathway to low-sugar functional beverages.Current research in food science · 2025Article
- β-Glucan Enhances the Biocontrol Efficacy of Marine YeastJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- Proteomic Analysis Explores Interactions betweenMicroorganisms · 2021Article
- Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies.International journal of molecular sciences · 2021Article
- Rewiring yeast metabolism to synthesize products beyond ethanol.Current opinion in chemical biology · 2020Review
- Corrigendum to "Rewiring yeast metabolism to synthesize products beyond ethanol" [Curr Opin Chem Biol 59 (December 2020) 182-192].Current opinion in chemical biology · 2020Article
- The Role of Yeasts and Lactic Acid Bacteria on the Metabolism of Organic Acids during Winemaking.Foods (Basel, Switzerland) · 2020Review
- Squalene-Tetrahymanol Cyclase Expression Enables Sterol-Independent Growth of Saccharomyces cerevisiae.Applied and environmental microbiology · 2020Article
- The Warburg Effect in Yeast: Repression of Mitochondrial Metabolism Is Not a Prerequisite to Promote Cell Proliferation.Frontiers in oncology · 2020Article
- HimalayanApplied and environmental microbiology · 2019Article
- Reassessment of requirements for anaerobic xylose fermentation by engineered, non-evolved Saccharomyces cerevisiae strains.FEMS yeast research · 2019Article
- Eight Kinetically Stable but Thermodynamically Activated Molecules that Power Cell Metabolism.Chemical reviews · 2018Review
- Structural, Physiological and Regulatory Analysis of Maltose Transporter Genes inFrontiers in microbiology · 2018Article
- Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in SBiotechnology for biofuels · 2017Article
- Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyruvate and acetyl-coenzyme A, located at the interface between glycolysis and TCA cycle, are important intermediates in yeast metabolism and key precursors for industrially relevant products. Rational engineering of their supply requires knowledge of compensatory reactions that replace predominant pathways when these are inactivated. This study investigates effects of individual and combined mutations that inactivate the mitochondrial pyruvate-dehydrogenase (PDH) complex, extramitochondrial citrate synthase (Cit2) and mitochondrial CoA-transferase (Ach1) in Saccharomyces cerevisiae. Additionally, strains with a constitutively expressed carnitine shuttle were constructed and analyzed. A predominant role of the PDH complex in linking glycolysis and TCA cycle in glucose-grown batch cultures could be functionally replaced by the combined activity of the cytosolic PDH bypass and Cit2. Strongly impaired growth and a high incidence of respiratory deficiency in pda1Δ ach1Δ strains showed that synthesis of intramitochondrial acetyl-CoA as a metabolic precursor requires activity of either the PDH complex or Ach1. Constitutive overexpression of AGP2, HNM1, YAT2, YAT1, CRC1 and CAT2 enabled the carnitine shuttle to efficiently link glycolysis and TCA cycle in l-carnitine-supplemented, glucose-grown batch cultures. Strains in which all known reactions at the glycolysis-TCA cycle interface were inactivated still grew slowly on glucose, indicating additional flexibility at this key metabolic junction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.