ArticleJournal of experimental botany2016
PME58 plays a role in pectin distribution during seed coat mucilage extrusion through homogalacturonan modification.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 59 citations in OpenAlex.
- Pectin Structural Dynamics: Developmental and Evolutionary Perspectives.Plants (Basel, Switzerland) · 2026Review
- Seed Mucilage Polysaccharides: A Comprehensive Review of Molecular Architecture, Green Extraction Technologies, Bioactivities, and Industrial Applications.Foods (Basel, Switzerland) · 2026Review
- OsvWA36 transcriptionally regulates cell wall remodeling to control grain length in rice.BMC plant biology · 2026Article
- Advances in the functional research of pectin methylesterases in Arabidopsis thaliana.BMC plant biology · 2026Review
- Holistic overview of regulatory networks governing seed dormancy and germination in plants.Science China. Life sciences · 2026Review
- Natural variation in FtPME58 contributes to seed hull thickness of Tartary buckwheat.Plant communications · 2026Article
- Mucilicious methods: Navigating the tools developed to Arabidopsis Seed Coat Mucilage analysis.Cell surface (Amsterdam, Netherlands) · 2025Review
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- Article
- The transcription factors ZAT5 and BLH2/4 regulate homogalacturonan demethylesterification in Arabidopsis seed coat mucilage.The Plant cell · 2024Article
- Pectin Remodeling and Involvement of AtPME3 in the Parasitic Plant-Plant Interaction,Plants (Basel, Switzerland) · 2024Article
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- Mucilage extracted from Chilean papaya seeds is enriched with homogalacturonan domains.Frontiers in plant science · 2024Article
- Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore Development.Plants (Basel, Switzerland) · 2023Article
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- The evolving views of the simplest pectic polysaccharides: homogalacturonan.Plant cell reports · 2022Review
- Genome-wide identification of PME gene family and expression of candidate genes associated with aluminum tolerance in tea plant (Camellia sinensis).BMC plant biology · 2022Article
- Systematic analysis of the pectin methylesterase gene family inFrontiers in plant science · 2022Article
- The Plant Invertase/Pectin Methylesterase Inhibitor Superfamily.Frontiers in plant science · 2022Review
- Conserved co-functional network between maize and Arabidopsis aid in the identification of seed defective genes in maize.Genes & genomics · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pectins are major components of plant primary cell walls. They include homogalacturonans (HGs), which are the most abundant pectin and can be the target of apoplastic enzymes like pectin methylesterases (PMEs) that control their methylesterification level. Several PMEs are expressed in the seed coat of Arabidopsis thaliana, particularly in mucilage secretory cells (MSCs). On the basis of public transcriptomic data, seven PME genes were selected and checked for their seed-specific expression by quantitative reverse transcription PCR. Of these, PME58 presented the highest level of expression and was specifically expressed in MSCs at the early stages of seed development. pme58 mutants presented two discrete phenotypes: (i) their adherent mucilage was less stained by ruthenium red when compared to wild-type seeds, but only in the presence of EDTA, a Ca(2+)chelator; and (ii) the MSC surface area was decreased. These phenotypes are the consequence of an increase in the degree of HG methylesterification connected to a decrease in PME activity. Analysis of the sugar composition of soluble and adherent mucilage showed that, in the presence of EDTA, sugars of adherent mucilage were more readily extracted in pme58 mutants. Immunolabelling with LM19, an antibody that preferentially recognizes unesterified HGs, also showed that molecular interactions with HGs were modified in the adherent mucilage of pme58 mutants, suggesting a role of PME58 in mucilage structure and organization. In conclusion, PME58 is the first PME identified to play a direct role in seed mucilage structure.
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Registered trials
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