ArticleActa crystallographica. Section D, Structural biology2016
High-density grids for efficient data collection from multiple crystals.
Article in Acta crystallographica. Section D, Structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 75 citations in OpenAlex.
- A simple and practical approach for protein serial crystallography using grease matrix and large-area support film.Journal of synchrotron radiation · 2026Article
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- Review
- Novel fixed-target serial crystallography flip-holder for macromolecular crystallography beamlines at synchrotron radiation sources.Journal of synchrotron radiation · 2025Article
- Scalable fabrication of an array-type fixed-target device for automated room temperature X-ray protein crystallography.Scientific reports · 2025Article
- Deep residual networks for crystallography trained on synthetic data.Acta crystallographica. Section D, Structural biology · 2024Article
- All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.Biomicrofluidics · 2023Article
- Growing and making nano- and microcrystals.Nature protocols · 2023Review
- Capturing the sequence of events during the water oxidation reaction in photosynthesis using XFELs.FEBS letters · 2023Review
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography.Lab on a chip · 2021Article
- Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Advances in methods for atomic resolution macromolecular structure determination.F1000Research · 2020Review
- The crystal structure of dGTPase reveals the molecular basis of dGTP selectivity.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography.Scientific reports · 2019Article
- Crystal-on-crystal chips for in situ serial diffraction at room temperature.Lab on a chip · 2018Article
- XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Bright Future for Serial Femtosecond Crystallography with XFELs.Trends in biochemical sciences · 2017Review
- High-speed fixed-target serial virus crystallography.Nature methods · 2017Article
Corrections and comments
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Authors and funding
33 authors at 11 institutions in 3 countries.
Funding
Abstract
Higher throughput methods to mount and collect data from multiple small and radiation-sensitive crystals are important to support challenging structural investigations using microfocus synchrotron beamlines. Furthermore, efficient sample-delivery methods are essential to carry out productive femtosecond crystallography experiments at X-ray free-electron laser (XFEL) sources such as the Linac Coherent Light Source (LCLS). To address these needs, a high-density sample grid useful as a scaffold for both crystal growth and diffraction data collection has been developed and utilized for efficient goniometer-based sample delivery at synchrotron and XFEL sources. A single grid contains 75 mounting ports and fits inside an SSRL cassette or uni-puck storage container. The use of grids with an SSRL cassette expands the cassette capacity up to 7200 samples. Grids may also be covered with a polymer film or sleeve for efficient room-temperature data collection from multiple samples. New automated routines have been incorporated into the Blu-Ice/DCSS experimental control system to support grids, including semi-automated grid alignment, fully automated positioning of grid ports, rastering and automated data collection. Specialized tools have been developed to support crystallization experiments on grids, including a universal adaptor, which allows grids to be filled by commercial liquid-handling robots, as well as incubation chambers, which support vapor-diffusion and lipidic cubic phase crystallization experiments. Experiments in which crystals were loaded into grids or grown on grids using liquid-handling robots and incubation chambers are described. Crystals were screened at LCLS-XPP and SSRL BL12-2 at room temperature and cryogenic temperatures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.