ArticleOncoTargets and therapy2016
Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol.
Article in OncoTargets and therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Beyond Milk and Nurture: Breastfeeding's Powerful Impact on Breast Cancer.Geburtshilfe und Frauenheilkunde · 2024Article
- Leptin, Adiponectin, and Melatonin Modulate Colostrum Lymphocytes in Mothers with Obesity.International journal of molecular sciences · 2023Article
- The role of breastfeeding in breast cancer prevention: a literature review.Frontiers in oncology · 2023Review
- Effects of Modified Melatonin Release on Human Colostrum Neutrophils to Induce Death in the MCF-7 Cell Line.International journal of cell biology · 2022Article
- Melatonin Hormone Acts on Cells of Maternal Blood and Placenta From Diabetic Mothers.Frontiers in physiology · 2021Article
- Article
- Melatonin Action on the Activity of Phagocytes from the Colostrum of Obese Women.Medicina (Kaunas, Lithuania) · 2019Article
- Effects of Mangaba (Hancornia speciosa) Fruit Extract Adsorbed onto PEG Microspheres in MCF-7 Breast Cancer Cells Co-Cultured with Blood Cells.Asian Pacific journal of cancer prevention : APJCP · 2019Article
- Article
- Zika Virus Alters the Viscosity and Cytokines Profile in Human Colostrum.Journal of immunology research · 2019Article
- High salt induces P-glycoprotein mediated treatment resistance in breast cancer cells through store operated calcium influx.Oncotarget · 2018Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeClinical and epidemiological studies have indicated that breastfeeding has a protective effect on breast cancer risk. Protein-based drugs, including antibodies, are being developed to attain better forms of cancer therapy. Secretory IgA (SIgA) is the antibody class in human breast milk, and its activity can be linked to the protective effect of breastfeeding. The aim of this study was to investigate the effect of polyethylene glycol (PEG) microspheres with adsorbed SIgA on MCF-7 human breast cancer cells.
methodsThe PEG microspheres were characterized by flow cytometry and fluorescence microscopy. The MCF-7 cells were obtained from American Type Culture Collection. MCF-7 cells were pre-incubated for 24 hours with or without SIgA (100 ng/mL), PEG microspheres or SIgA adsorbed in PEG microspheres (100 ng/mL). Viability, intracellular calcium release, and apoptosis in MCF-7 cells were determined by flow cytometry.
resultsFluorescence microscopy and flow cytometry analyses revealed that SIgA was able to adsorb to the PEG microspheres. The MCF-7 cells that were incubated with PEG microspheres with adsorbed SIgA showed decreased viability. MCF-7 cells that were incubated with SIgA or PEG microspheres with adsorbed SIgA had increased intracellular Ca(2+) levels. In the presence of SIgA, an increase in the percentage of apoptotic cells was observed. The highest apoptosis index was observed when the cells were treated with PEG microspheres with adsorbed SIgA.
conclusionThese data suggest that colostral SIgA adsorbed to PEG microspheres has antitumor effects on human MCF-7 breast cancer cells and that the presence of large amounts of this protein in secreted breast milk may provide protection against breast tumors in women who breastfed.
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