ArticleScientific reports2016
A versatile strategy for rapid conditional genome engineering using loxP sites in a small synthetic intron in Plasmodium falciparum.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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77 citing papers in PubMed, 138 citations in OpenAlex.
- Characterization of Sec14 Domain-Containing Proteins in the Malaria Parasite Plasmodium falciparum.Molecular microbiology · 2026Article
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- Physiological febrile heat stress increases cytoadhesion through increased protein trafficking ofeLife · 2026Article
- The fast-evolving FIKK kinase family of Plasmodium falciparum can be inhibited by a single compound.Nature microbiology · 2025Article
- PI3-kinase has multiple functions in asexual blood stages of Plasmodium falciparum.Scientific reports · 2025Article
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- PfFBXO1 is essential for inner membrane complex formation in Plasmodium falciparum during both asexual and transmission stages.Communications biology · 2025Article
- Constitutive expression of Cas9 and rapamycin-inducible Cre recombinase facilitates conditional genome editing in Plasmodium berghei.Scientific reports · 2025Article
- ThemSphere · 2024Article
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- Generation of a new DiCre expressing parasite strain for functional characterization of Plasmodium falciparum genes in blood stages.Scientific reports · 2024Article
- The threemSphere · 2024Article
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- Genetic validation ofeLife · 2023Article
- DNA-binding protein PfAP2-P regulates parasite pathogenesis during malaria parasite blood stages.Nature microbiology · 2023Article
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- TheAntimicrobial agents and chemotherapy · 2023Article
- Knock-sideways by inducible ER retrieval enables a unique approach for studyingProceedings of the National Academy of Sciences of the United States of America · 2023Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
Conditional genome engineering in the human malaria pathogen Plasmodium falciparum remains highly challenging. Here we describe a strategy for facile and rapid functional analysis of genes using an approach based on the Cre/lox system and tailored for organisms with short and few introns. Our method allows the conditional, site-specific removal of genomic sequences of essential and non-essential genes by placing loxP sites into a short synthetic intron to produce a module (loxPint) can be placed anywhere in open reading frames without compromising protein expression. When duplicated, the loxPint module serves as an intragenic recombineering point that can be used for the fusion of gene elements to reporters or the conditional introduction of point mutations. We demonstrate the robustness and versatility of the system by targeting the P. falciparum merozoite surface protein 1 gene (msp1), which has previously proven refractory to genetic interrogation, and the parasite exported kinase FIKK10.1.
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