ArticleBiology open2016
Genetic basis of hindlimb loss in a naturally occurring vertebrate model.
Article in Biology open, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Genome of Kumamoto OysterEvolutionary applications · 2025Article
- Novel function of Hox13 in regulating outgrowth of the newt hindlimb bud through interaction with Fgf10 and Tbx4.Development, growth & differentiation · 2025Article
- The functional roles of zebrafish HoxA- and HoxD-related clusters in the pectoral fin development.Scientific reports · 2024Article
- Lateral thinking in syndromic congenital cardiovascular disease.Disease models & mechanisms · 2023Article
- Molecular Function and Contribution ofAmerican journal of respiratory and critical care medicine · 2023Review
- Heterochronic development of pelvic fins in zebrafish: possible involvement of temporal regulation ofFrontiers in cell and developmental biology · 2023Article
- Identification and Functional Evaluation of a NovelInternational journal of molecular sciences · 2022Article
- (Non)Parallel developmental mechanisms in vertebrate appendage reduction and loss.Ecology and evolution · 2021Review
- Fgf-driven Tbx protein activities directly induceDevelopment (Cambridge, England) · 2020Article
- Homozygous Null TBX4 Mutations Lead to Posterior Amelia with Pelvic and Pulmonary Hypoplasia.American journal of human genetics · 2019Article
- Pigeon foot feathering reveals conserved limb identity networks.Developmental biology · 2019Article
- Genome Sequencing of the Japanese Eel (Marine drugs · 2019Article
- Limb development: a paradigm of gene regulation.Nature reviews. Genetics · 2017Review
- The origins, scaling and loss of tetrapod digits.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- The genome of the Gulf pipefish enables understanding of evolutionary innovations.Genome biology · 2016Article
- Article
- Developmental Mechanism of Limb Field Specification along the Anterior-Posterior Axis during Vertebrate Evolution.Journal of developmental biology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we genetically characterise pelvic finless, a naturally occurring model of hindlimb loss in zebrafish that lacks pelvic fin structures, which are homologous to tetrapod hindlimbs, but displays no other abnormalities. Using a hybrid positional cloning and next generation sequencing approach, we identified mutations in the nuclear localisation signal (NLS) of T-box transcription factor 4 (Tbx4) that impair nuclear localisation of the protein, resulting in altered gene expression patterns during pelvic fin development and the failure of pelvic fin development. Using a TALEN-induced tbx4 knockout allele we confirm that mutations within the Tbx4 NLS (A78V; G79A) are sufficient to disrupt pelvic fin development. By combining histological, genetic, and cellular approaches we show that the hindlimb initiation gene tbx4 has an evolutionarily conserved, essential role in pelvic fin development. In addition, our novel viable model of hindlimb deficiency is likely to facilitate the elucidation of the detailed molecular mechanisms through which Tbx4 functions during pelvic fin and hindlimb development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.