ArticleJournal of clinical laboratory analysis2016
Detection of Treponema pallidum Sp. Pallidum DNA in Cerebrospinal Fluid (CSF) by Two PCR Techniques.
Article in Journal of clinical laboratory analysis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Global research trends in neurosyphilis: a bibliometric analysis from 2010 to 2024.Frontiers in immunology · 2025Pooled it
- Diagnostic tools for neurosyphilis: a systematic review.BMC infectious diseases · 2021Pooled it
- Rapid visual detection of Treponema pallidum using the RPA-CRISPR/Cas12a system.Scientific reports · 2026Article
- Update on syphilis diagnostics.Current opinion in infectious diseases · 2025Review
- Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.Clinical microbiology reviews · 2024Review
- Canadian Public Health Laboratory Network (CPHLN) Diagnostic Recommendations for Neurosyphilis in Canada.Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 2024Article
- Loop-Mediated Isothermal Amplification Assay Using Gold Nanoparticles for DetectingDiagnostics (Basel, Switzerland) · 2024Article
- CDC Laboratory Recommendations for Syphilis Testing, United States, 2024.MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports · 2024Article
- Neurosyphilis with sequential respiratory complications: A case report highlighting diagnostic and treatment challenges.SAGE open medical case reports · 2024Article
- Secondary immunoreaction in patients with neurosyphilis and its relevance to clinical outcomes.Frontiers in neurology · 2023Article
- Intrathecal Synthesis Index of Specific Anti-Microbiology spectrum · 2022Article
- Advances in laboratory diagnostic methods for cerebrospinal fluid testing for neurosyphilis.Frontiers in public health · 2022Review
- Immune Privilege Furnishes a Niche for Latent Infection.Frontiers in ophthalmology · 2022Review
- Laboratory Perspective on Racial Disparities in Sexually Transmitted Infections.The journal of applied laboratory medicine · 2021Review
- Molecular and Direct Detection Tests for Treponema pallidum Subspecies pallidum: A Review of the Literature, 1964-2017.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Review
- Laboratory Diagnostic Tools for Syphilis: Current Status and Future Prospects.Frontiers in cellular and infection microbiology · 2020Review
- PCR detection for syphilis diagnosis: Status and prospects.Journal of clinical laboratory analysis · 2019Review
- Gene subtype analysis ofExperimental and therapeutic medicine · 2018Article
- Syphilis.Nature reviews. Disease primers · 2017Review
- Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLaboratory diagnosis of neurosyphilis is complicated especially when it is asymptomatic, no single laboratory test result being appropriate to diagnose central nervous system infectivity caused by Treponema pallidum. Our objective was to evaluate two polymerase chain reaction (PCR) techniques for the detection of T. pallidum DNA in the cerebrospinal fluid (CSF) of patients with syphilis.
methodsOne hundred twenty-four CSF samples from patients with reactive blood tests for syphilis were obtained. Two PCR techniques (47-PCR, polA-PCR) were used to detect T. pallidum DNA. The laboratory criteria used for the diagnosis of neurosyphilis to which the PCR techniques were compared were those recommended by the IUSTI: 2008 European guidelines on the management of syphilis.
resultsTreponema pallidum DNA was detected amplified in 37 of 124 (29.8%) and 30 of 124 (24.2%) samples with the 47-PCR and polA-PCR, respectively. Sensitivities were 75.8% and 69.7% and specificities 86.8% and 92.3%, respectively, for 47-PCR and polA-PCR techniques, respectively. The three CSF samples of patients with primary syphilis did not fulfill the criteria of neurosyphilis and DNA was only detected in one by the 47-PCR. In samples from secondary syphilis and neurosyphilis, three of nine and nine of nine respectively, results were coincident for the two PCR techniques and neurosyphilis criteria. Major discrepancies between the two PCR techniques and neurosyphilis diagnostic criteria were observed in latent syphilis.
conclusionBeyond some limitations of the study, which are discussed here, both PCR techniques seem to be useful for the diagnosis of neurosyphilis, although 47-PCR presents a higher sensitivity and polA-PCR a higher specificity.
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