Evidence map›Paper›PMID 26892227›Full record

ArticleEuropean spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society2017

The effect of capacitively coupled (CC) electrical stimulation on human disc nucleus pulposus cells and the relationship between CC and BMP-7.

Zili Wang, William C Hutton, S Tim Yoon

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In one paragraph

Article in European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Integration of clinical perspective into biomimetic bioreactor design for orthopedics.Journal of biomedical materials research. Part B, Applied biomaterials · 2022
    Review
  5. Intervertebral Disc-on-a-Chip as AdvancedFrontiers in bioengineering and biotechnology · 2021
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Zili WangThe Atlanta Department of Veterans Affairs Medical Center, The Emory Spine Center, Emory University School of Medicine, Atlanta, GA, USA. zwang2@emory.edu.
William C HuttonThe Atlanta Department of Veterans Affairs Medical Center, The Emory Spine Center, Emory University School of Medicine, Atlanta, GA, USA.
S Tim YoonThe Atlanta Department of Veterans Affairs Medical Center, The Emory Spine Center, Emory University School of Medicine, Atlanta, GA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeAn in vitro study using human intervertebral disc nucleus pulposus cells to evaluate the effects of CC stimulation on disc-matrix macromolecule production.

methodsNucleus pulposus cells were cultured in alginate beads and treated with CC stimulation. The effect of BMP on CC stimulation of the cells was evaluated by applying a BMP blocker (noggin) or by applying additional BMP-7 to the culture. The mRNA levels of the disc extracellular matrix genes (collagen I, II, aggrecan) and BMPs were measured by real-time PCR. The protein levels of aggrecan, collagen II, and BMPs were determined by ELISAs and Western blots. Sulfated glycosaminoglycan (sGAG) content was assayed using the DMMB method.

results(1) CC stimulation upregulates the production of the disc-matrix macromolecular components: sGAG, aggrecan and collagen II; (2) CC stimulation increases the ratio of mRNA expression levels of collagen II to collagen I; (3) CC stimulation induces the expression of endogenous BMP-4 and BMP-7; (4) inhibition of BMP activity (using noggin) reduces CC-mediated upregulation of aggrecan and collagen II; (5) CC and BMP-7 act in synergy to increase the upregulation of disc-matrix macromolecules.

conclusionCC stimulation upregulates the production of the intervertebral disc-matrix macromolecules aggrecan, collagen II, and sGAG by a mechanism involving BMPs. CC stimulation acts in synergy with BMP-7 to increase the upregulation of these disc-matrix macromolecules.

Indexed as

AggrecansBone Morphogenetic Protein 4Bone Morphogenetic Protein 7Cells, CulturedCollagen Type ICollagen Type IIElectric StimulationGlycosaminoglycansHumansIntervertebral DiscNucleus PulposusReal-Time Polymerase Chain ReactionRNA, MessengerUp-RegulationAggrecansBMP4 protein, humanBMP7 protein, humanBone Morphogenetic Protein 4Bone Morphogenetic Protein 7Collagen Type ICollagen Type IIGlycosaminoglycansRNA, MessengerAggrecanBMP-7Capacitively coupled electrical stimulationDisc cell biologyDisc degeneration

Identifiers

PMID26892227

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.