ArticleScientific reports2016
Purity matters: A workflow for the valid high-resolution lipid profiling of mitochondria from cell culture samples.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
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- Purification of murine cortex mitochondria using a modified magnetic bead isolation method.MethodsX · 2026Article
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- Mitochondrial Metabolomics in Cancer: Mass Spectrometry-Based Approaches for Metabolic Rewiring Analysis and Therapeutic Discovery.Metabolites · 2025Review
- Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Cytidine deaminase-dependent mitochondrial biogenesis as a potential vulnerability in pancreatic cancer cells.Communications biology · 2024Article
- Mitochondrial phosphoproteomes are functionally specialized across tissues.Life science alliance · 2024Article
- The expanding organelle lipidomes: current knowledge and challenges.Cellular and molecular life sciences : CMLS · 2023Review
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- Exercise prevents fatty liver by modifying the compensatory response of mitochondrial metabolism to excess substrate availability.Molecular metabolism · 2021Article
- Dystrophin Dp71 Subisoforms Localize to the Mitochondria of Human Cells.Life (Basel, Switzerland) · 2021Article
- Understanding intracellular nanoparticle trafficking fates through spatiotemporally resolved magnetic nanoparticle recovery.Nanoscale advances · 2021Review
- Methodological Issue of Mitochondrial Isolation in Acute-Injury Rat Model: Asphyxia Cardiac Arrest and Resuscitation.Frontiers in medicine · 2021Article
- Isolation and Metabolic Assessment of Cancer Cell Mitochondria.ACS omega · 2020Article
- Novel approach to quantify mitochondrial content and intrinsic bioenergetic efficiency across organs.Scientific reports · 2020Article
- A quantitative LC-MS/MS method for analysis of mitochondrial -specific oxysterol metabolism.Redox biology · 2020Article
- Novel mitochondrial and cytosolic purification pipeline for compartment-specific metabolomics in mammalian disease model tissues.Metabolomics : Official journal of the Metabolomic Society · 2020Article
- Mitochondrial Ceramide Effects on the Retinal Pigment Epithelium in Diabetes.International journal of molecular sciences · 2020Article
- Metabo-lipidomics of Fibroblasts and Mitochondrial-Endoplasmic Reticulum Extracts from ALS Patients Shows Alterations in Purine, Pyrimidine, Energetic, and Phospholipid Metabolisms.Molecular neurobiology · 2019Article
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Subcellular lipidomics is a novel field of research that requires the careful combination of several pre-analytical and analytical steps. To define a reliable strategy for mitochondrial lipid profiling, we performed a systematic comparison of different mitochondria isolation procedures by western blot analyses and comprehensive high-resolution lipidomics. Using liver-derived HepG2 cells, we compared three common mitochondria isolation methods, differential centrifugation (DC), ultracentrifugation (UC) and a magnetic bead-assisted method (MACS). In total, 397 lipid species, including 32 cardiolipins, could be quantified in only 100 μg (by protein) of purified mitochondria. Mitochondria isolated by UC showed the highest enrichment in the mitochondria-specific cardiolipins as well as their precursors, phosphatidylglycerols. Mitochondrial fractions obtained by the commonly used DC and the more recent MACS method contained substantial contaminations by other organelles. Employing these isolation methods when performing lipidomics analyses from cell culture mitochondria may lead to inaccurate results. To conclude, we present a protocol how to obtain reliable mitochondria-specific lipid profiles from cell culture samples and show that quality controls are indispensable when performing mitochondria lipidomics.
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