ArticleScientific reports2016
Combined strategies for optimal detection of the contact point in AFM force-indentation curves obtained on thin samples and adherent cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 94 citations in OpenAlex.
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- High-Speed Quantitative Nanomechanical Mapping by Photothermal Off-Resonance Atomic Force Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
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- Automated atomic force microscopy analysis using convolutional and recurrent neural networks.Biophysical journal · 2025Article
- Epithelial Cell Mechanoresponse to Matrix Viscoelasticity and Confinement Within Micropatterned Viscoelastic Hydrogels.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Regional Variations in Mechanical Properties of Porcine Leptomeninges.Cyborg and bionic systems (Washington, D.C.) · 2025Article
- Side-view optical microscopy-assisted atomic force microscopy for thickness-dependent nanobiomechanics.Nanoscale advances · 2024Article
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- Atomic Force Microscopy for the Study of Cell Mechanics in Pharmaceutics.Pharmaceutics · 2024Review
- Cryosectioning of Hydrogels as a Reliable Approach to Increase Yield and Further Tune Mechanical Properties.Gels (Basel, Switzerland) · 2023Article
- Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy.STAR protocols · 2022Article
- Force Sensing on Cells and Tissues by Atomic Force Microscopy.Sensors (Basel, Switzerland) · 2022Article
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy.Nanomaterials (Basel, Switzerland) · 2021Article
- Determination by Relaxation Tests of the Mechanical Properties of Soft Polyacrylamide Gels Made for Mechanobiology Studies.Polymers · 2021Article
- The Mechanical Interplay Between Differentiating Mesenchymal Stem Cells and Gelatin-Based Substrates Measured by Atomic Force Microscopy.Frontiers in cell and developmental biology · 2021Article
- Effects of energy metabolism on the mechanical properties of breast cancer cells.Communications biology · 2020Article
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Authors and funding
1 author at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Atomic Force Microscopy (AFM) is a widely used tool to study cell mechanics. Current AFM setups perform high-throughput probing of living cells, generating large amounts of force-indentations curves that are subsequently analysed using a contact-mechanics model. Here we present several algorithms to detect the contact point in force-indentation curves, a crucial step to achieve fully-automated analysis of AFM-generated data. We quantify and rank the performance of our algorithms by analysing a thousand force-indentation curves obtained on thin soft homogeneous hydrogels, which mimic the stiffness and topographical profile of adherent cells. We take advantage of the fact that all the proposed algorithms are based on sequential search strategies, and show that a combination of them yields the most accurate and unbiased results. Finally, we also observe improved performance when force-indentation curves obtained on adherent cells are analysed using our combined strategy, as compared to the classical algorithm used in the majority of previous cell mechanics studies.
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