ArticleBioengineered2016
Activation of futile cycles as an approach to increase ethanol yield during glucose fermentation in Saccharomyces cerevisiae.
Article in Bioengineered, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 29 citations in OpenAlex.
- Improvement of ethanol and 2,3-butanediol production in Saccharomyces cerevisiae by ATP wasting.Microbial cell factories · 2023Article
- FromJournal of fungi (Basel, Switzerland) · 2023Review
- Principles and practice of designing microbial biocatalysts for fuel and chemical production.Journal of industrial microbiology & biotechnology · 2022Review
- Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production.Synthetic and systems biotechnology · 2022Article
- Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production in Clostridium acetobutylicum.Microbial cell factories · 2021Article
- ATPase-based implementation of enforced ATP wasting in Saccharomyces cerevisiae for improved ethanol production.Biotechnology for biofuels · 2020Article
- Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).Journal of industrial microbiology & biotechnology · 2020Review
- A synthetic medium to simulate sugarcane molasses.Biotechnology for biofuels · 2018Article
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4 authors at 3 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
An increase in ethanol yield by yeast from the fermentation of conventional sugars such as glucose and sucrose is possible by reducing the production of a key byproduct such as cellular biomass. Previously we have reported that overexpression of PHO8 gene encoding non-specific ATP-hydrolyzing alkaline phosphatase can lead to a decrease in cellular ATP content and to an increase in ethanol yield during glucose fermentation by Saccharomyces cerevisiae. In this work we further report on 2 new successful approaches to reduce cellular levels of ATP that increase ethanol yield and productivity. The first approach is based on the overexpression of the heterologous Escherichia coli apy gene encoding apyrase or SSB1 part of the chaperon that exhibit ATPase activity in yeast. In the second approach we constructed a futile cycle by the overexpression of S. cerevisiae genes encoding pyruvate carboxylase and phosphoenolpyruvate carboxykinase in S. cerevisiae. These genetically engineered strains accumulated more ethanol compared to the wild-type strain during alcoholic fermentation.
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