ArticleNature protocols2016
A cryoinjury model in neonatal mice for cardiac translational and regeneration research.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed, 48 citations in OpenAlex.
- Heart ventricle regeneration in the lizard Eublepharis macularius, the leopard gecko.NPJ Regenerative medicine · 2026Article
- Cauterization of the root of the left coronary artery as a straightforward, large and reproducible ischemic injury model in neonatal mice.Lab animal · 2024Review
- Murine neonatal cardiac regeneration depends on Insulin-like growth factor 1 receptor signaling.Scientific reports · 2024Article
- Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions.Nature communications · 2024Article
- Heart-on-a-Chip Model of Epicardial-Myocardial Interaction in Ischemia Reperfusion Injury.Advanced healthcare materials · 2024Article
- Effects and mechanisms of the myocardial microenvironment on cardiomyocyte proliferation and regeneration.Frontiers in cell and developmental biology · 2024Review
- Coupled myovascular expansion directs cardiac growth and regeneration.Development (Cambridge, England) · 2022Article
- Cardiovascular Diseases in the Digital Health Era: A Translational Approach from the Lab to the Clinic.Biotech (Basel (Switzerland)) · 2022Review
- Characterizing Neonatal Heart Maturation, Regeneration, and Scar Resolution Using Spatial Transcriptomics.Journal of cardiovascular development and disease · 2021Article
- Protocol for cryoinjury model in neonatal mice for heart regeneration and repair research.STAR protocols · 2021Article
- Review
- GLUT1 overexpression enhances glucose metabolism and promotes neonatal heart regeneration.Scientific reports · 2021Article
- Celecoxib alleviates pathological cardiac hypertrophy and fibrosis via M1-like macrophage infiltration in neonatal mice.iScience · 2021Article
- A surgical mouse model of neonatal pressure overload by transverse aortic constriction.Nature protocols · 2021Article
- Clonal Analysis of the Neonatal Mouse Heart using Nearest Neighbor Modeling.Journal of visualized experiments : JoVE · 2020Article
- Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury.Cell reports · 2019Article
- Cardiomyocyte cell cycle dynamics and proliferation revealed through cardiac-specific transgenesis of fluorescent ubiquitinated cell cycle indicator (FUCCI).Journal of molecular and cellular cardiology · 2019Article
- Zebrafish as a Smart Model to Understand Regeneration After Heart Injury: How Fish Could Help Humans.Frontiers in cardiovascular medicine · 2019Review
- New Myocyte Formation in the Adult Heart: Endogenous Sources and Therapeutic Implications.Circulation research · 2018Review
- Dusp6 attenuates Ras/MAPK signaling to limit zebrafish heart regeneration.Development (Cambridge, England) · 2018Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
The introduction of injury models for neonatal mouse hearts has accelerated research on the mechanisms of cardiac regeneration in mammals. However, some existing models, such as apical resection and ligation of the left anterior descending artery, produce variable results, which may be due to technical difficulties associated with these methods. Here we present an alternative model for the study of cardiac regeneration in neonatal mice in which cryoinjury is used to induce heart injury. This model yields a reproducible injury size, does not induce known mechanisms of cardiac regeneration and leads to a sustained reduction of cardiac function. This protocol uses reusable cryoprobes that can be assembled in 5 min, with the entire procedure taking 15 min per pup. The subsequent heart collection and fixation takes 2 d to complete. Cryoinjury results in a myocardial scar, and the size of injury can be scaled by the use of different cryoprobes (0.5 and 1.5 mm). Cryoinjury models are medically relevant to diseases in human infants with heart disease. In summary, the myocardial cryoinjury model in neonatal mice described here is a useful tool for cardiac translational and regeneration research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.