ArticlePLoS biology2016
Gβ Regulates Coupling between Actin Oscillators for Cell Polarity and Directional Migration.
Article in PLoS biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Inwardly rectifying potassium channels promote directional sensing during neutrophil chemotaxis.The Journal of cell biology · 2026Article
- Inwardly rectifying potassium channels regulate membrane potential polarization and direction sensing during neutrophil chemotaxis.bioRxiv : the preprint server for biology · 2025Article
- Roadmap on biology in time varying environments.Physical biology · 2021Review
- Structural Basis of CYRI-B Direct Competition with Scar/WAVE Complex for Rac1.Structure (London, England : 1993) · 2021Article
- Pattern formation in reaction-diffusion system on membrane with mechanochemical feedback.Scientific reports · 2020Article
- Review
- A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control.Cell systems · 2020Article
- A bright future: optogenetics to dissect the spatiotemporal control of cell behavior.Current opinion in chemical biology · 2019Review
- G-protein-coupled formyl peptide receptors play a dual role in neutrophil chemotaxis and bacterial phagocytosis.Molecular biology of the cell · 2019Article
- Control of actin dynamics during cell motility.F1000Research · 2019Review
- Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation.Nature communications · 2018Article
- Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.Nature cell biology · 2018Article
- Joining forces: crosstalk between biochemical signalling and physical forces orchestrates cellular polarity and dynamics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Rhythmicity and waves in the cortex of single cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Insight from the maximal activation of the signal transduction excitable network inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Excitable Signal Transduction Networks in Directed Cell Migration.Annual review of cell and developmental biology · 2017Review
- Oscillatory signatures underlie growth regimes in Arabidopsis pollen tubes: computational methods to estimate tip location, periodicity, and synchronization in growing cells.Journal of experimental botany · 2017Article
- Chemotactic network responses to live bacteria show independence of phagocytosis from chemoreceptor sensing.eLife · 2017Article
- Crosstalk of cell polarity signaling pathways.Protoplasma · 2017Review
- The Spatiotemporal Limits of Developmental Erk Signaling.Developmental cell · 2017Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
For directional movement, eukaryotic cells depend on the proper organization of their actin cytoskeleton. This engine of motility is made up of highly dynamic nonequilibrium actin structures such as flashes, oscillations, and traveling waves. In Dictyostelium, oscillatory actin foci interact with signals such as Ras and phosphatidylinositol 3,4,5-trisphosphate (PIP3) to form protrusions. However, how signaling cues tame actin dynamics to produce a pseudopod and guide cellular motility is a critical open question in eukaryotic chemotaxis. Here, we demonstrate that the strength of coupling between individual actin oscillators controls cell polarization and directional movement. We implement an inducible sequestration system to inactivate the heterotrimeric G protein subunit Gβ and find that this acute perturbation triggers persistent, high-amplitude cortical oscillations of F-actin. Actin oscillators that are normally weakly coupled to one another in wild-type cells become strongly synchronized following acute inactivation of Gβ. This global coupling impairs sensing of internal cues during spontaneous polarization and sensing of external cues during directional motility. A simple mathematical model of coupled actin oscillators reveals the importance of appropriate coupling strength for chemotaxis: moderate coupling can increase sensitivity to noisy inputs. Taken together, our data suggest that Gβ regulates the strength of coupling between actin oscillators for efficient polarity and directional migration. As these observations are only possible following acute inhibition of Gβ and are masked by slow compensation in genetic knockouts, our work also shows that acute loss-of-function approaches can complement and extend the reach of classical genetics in Dictyostelium and likely other systems as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.