ArticlePloS one2016
Copper Nanoparticles and Copper Sulphate Induced Cytotoxicity in Hepatocyte Primary Cultures of Epinephelus coioides.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Antibacterial, Redox, Cytotoxic, and Ecotoxic Properties of New Sol-Gel Silica-Copper-Based Materials.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Assessing the Combined Toxicity of Silver and Copper Nanoparticles in Rainbow Trout (Oncorhynchus mykiss) Fingerlings.Biological trace element research · 2025Article
- Autofluorescence Quenching in Decellularized Plant Scaffolds for Tissue Engineering.Annals of biomedical engineering · 2025Article
- Copper Nanoparticles in Aquatic Environment: Release Routes and Oxidative Stress-Mediated Mechanisms of Toxicity to Fish in Various Life Stages and Future Risks.Current issues in molecular biology · 2025Review
- Evaluating the therapeutic potential of BSA-reduced mussel-derived selenium nanoparticles to mitigate copper sulfate-induced hepatic damage and neurodegeneration in a zebrafish model.Frontiers in genetics · 2025Article
- Residual hyperglycemia after successful treatment of a patient with severe copper sulfate poisoning.Journal of Zhejiang University. Science. B · 2024Article
- Antioxidant-related enzymes and peptides as biomarkers of metallic nanoparticles (eco)toxicity in the aquatic environment.Chemosphere · 2024Review
- Cuproptosis in cancer: biological implications and therapeutic opportunities.Cellular & molecular biology letters · 2024Review
- Remediation of Metal Oxide Nanotoxicity with a Functional Amyloid.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Review
- Quercetin Alleviates Toxicity Induced by High Levels of Copper in Porcine Follicular Granulosa Cells by Scavenging Reactive Oxygen Species and Improving Mitochondrial Function.Animals : an open access journal from MDPI · 2023Article
- Understanding Nanomaterial-Liver Interactions to Facilitate the Development of Safer Nanoapplications.Advanced materials (Deerfield Beach, Fla.) · 2022Review
- Changes of Gene Expression Patterns from Aquatic Organisms Exposed to Metal Nanoparticles.International journal of environmental research and public health · 2021Review
- Polydatin Beneficial Effects in Zebrafish Larvae Undergoing Multiple Stress Types.International journal of environmental research and public health · 2021Article
- Antitumor effect of copper nanoparticles on human breast and colon malignancies.Environmental science and pollution research international · 2021Article
- Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents.Scientific reports · 2020Article
- Hepatoprotective activity of raspberry ketone is mediatedToxicology research · 2019Article
- Pomegranate Juice Diminishes The Mitochondria-Dependent Cell Death And NF-kB Signaling Pathway Induced By Copper Oxide Nanoparticles On Liver And Kidneys Of Rats.International journal of nanomedicine · 2019Article
- The Toxicity Of Metallic Nanoparticles On Liver: The Subcellular Damages, Mechanisms, And Outcomes.International journal of nanomedicine · 2019Review
- Protective role of jaboticaba Plinia peruviana peel extract in copper-induced cytotoxicity in Allium cepa.Environmental science and pollution research international · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copper nanoparticles (Cu-NPs) were widely used in various industrial and commercial applications. The aim of this study was to analyze the cytotoxicity of Cu-NPs on primary hepatocytes of E.coioides compared with copper sulphate (CuSO4). Cultured cells were exposed to 0 or 2.4 mg Cu L-1 as CuSO4or Cu-NPs for 24-h. Results showed either form of Cu caused a dramatic loss in cell viability, more so in the CuSO4 than Cu-NPs treatment. Compared to control, either CuSO4 or Cu-NPs significantly increased reactive oxygen species(ROS) and malondialdehyde(MDA) concentration in hepatocytes by overwhelming total superoxide dismutase (T-SOD) activity, catalase(CAT) activity and glutathione(GSH) concentration. In addition, the antioxidative-related genes [SOD (Cu/Zn), SOD (Mn), CAT, GPx4] were also down-regulated. The apoptosis and necrosis percentage was significantly higher after the CuSO4 or Cu-NPs treatment than the control. The apoptosis was induced by the increased cytochrome c concentration in the cytosol and elevated caspase-3, caspase-8 and caspase-9 activities. Additionally, the apoptosis-related genes (p53, p38β and TNF-α) and protein (p53 protein) were up-regulated after the CuSO4 or Cu-NPs treatment, with CuSO4 exposure having a greater effect than Cu-NPs. In conclusion, Cu-NPs had similar types of toxic effects as CuSO4 on primary hepatocytes of E.coioides, but toxicity of CuSO4 was more severe than that of Cu-NPs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.