ArticleNeural regeneration research2015
In vitro model of the blood-brain barrier established by co-culture of primary cerebral microvascular endothelial and astrocyte cells.
Article in Neural regeneration research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- A systematic review and meta-analysis of the relationship between neuroinflammation and blood-brain barrier based onBiochemistry and biophysics reports · 2026Review
- Anti-Inflammatory Effects of Polyphenols in Brain Microvascular Endothelial Cells Stimulated with TNF-α.International journal of molecular sciences · 2026Article
- Multiple sclerosis: etiology in the context of neurovascular unit and immune system involvement and advancements withFrontiers in immunology · 2025Review
- Article
- GDNF and cAMP significantly enhanceHeliyon · 2024Article
- Establishment of a Four-Cell In Vitro Blood-Brain Barrier Model With Human Primary Brain Cells.Current protocols · 2024Article
- A novel 4-cell in-vitro blood-brain barrier model and its characterization by confocal microscopy and TEER measurement.Journal of neuroscience methods · 2023Article
- Experimental Models to Study the Functions of the Blood-Brain Barrier.Bioengineering (Basel, Switzerland) · 2023Review
- Establishing Co-Culture Blood-Brain Barrier Models for Different Neurodegeneration Conditions to Understand Its Effect on BBB Integrity.International journal of molecular sciences · 2023Article
- The role of SUMOylation in the neurovascular dysfunction after acquired brain injury.Frontiers in pharmacology · 2023Review
- Astrocytes and human artificial blood-brain barrier models.Bosnian journal of basic medical sciences · 2022Review
- Astrocytic responses to high glucose impair barrier formation in cerebral microvessel endothelial cells.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- Simple and efficient protocol to isolate and culture brain microvascular endothelial cells from newborn mice.Frontiers in cellular neuroscience · 2022Article
- A sporadic Alzheimer's blood-brain barrier model for developing ultrasound-mediated delivery of Aducanumab and anti-Tau antibodies.Theranostics · 2022Article
- Research Models of the Nanoparticle-Mediated Drug Delivery across the Blood-Brain Barrier.Tissue engineering and regenerative medicine · 2021Review
- SARS-CoV-2 crosses the blood-brain barrier accompanied with basement membrane disruption without tight junctions alteration.Signal transduction and targeted therapy · 2021Article
- Fibrillar Nanomembranes of Recombinant Spider Silk Protein Support Cell Co-culture in anACS biomaterials science & engineering · 2021Article
- Bioengineered microfluidic blood-brain barrier models in oncology research.Translational oncology · 2021Review
- Macrophage membrane-coated nanocarriers Co-Modified by RVG29 and TPP improve brain neuronal mitochondria-targeting and therapeutic efficacy in Alzheimer's disease mice.Bioactive materials · 2021Article
- Increase in Blood-Brain Barrier (BBB) Permeability Is Regulated by MMP3 via the ERK Signaling Pathway.Oxidative medicine and cellular longevity · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drugs for the treatment and prevention of nervous system diseases must permeate the blood-brain barrier to take effect. In vitro models of the blood-brain barrier are therefore important in the investigation of drug permeation mechanisms. However, to date, no unified method has been described for establishing a blood-brain barrier model. Here, we modified an in vitro model of the blood-brain barrier by seeding brain microvascular endothelial cells and astrocytes from newborn rats on a polyester Transwell cell culture membrane with 0.4-µm pores, and conducted transepithelial electrical resistance measurements, leakage tests and assays for specific blood-brain barrier enzymes. We show that the permeability of our model is as low as that of the blood-brain barrier in vivo. Our model will be a valuable tool in the study of the mechanisms of action of neuroprotective drugs.
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