ArticleScientific reports2016
Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers, 1 of them a synthesis that pooled it.
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Who cites it
98 citing papers in PubMed, 1 synthesis or guideline pooled it, 253 citations in OpenAlex.
- Horizon scanning of potential environmental applications of terrestrial animals, fish, algae and microorganisms produced by genetic modification, including the use of new genomic techniques.Frontiers in genome editing · 2024Pooled it
- Engineering monosex and sterile fish for food production: from conventional methods to CRISPR-based precision.Advanced biotechnology · 2026Review
- Advances in Fish Gene Editing.Animals : an open access journal from MDPI · 2026Review
- Molecular insights into insulin-like signaling and its positive role in growth of the bivalveMarine life science & technology · 2026Article
- Intra-ovarian busulfan administration: an effective and relatively safe method for depleting endogenous germ cells in the female marine teleost yellow drum (Nibea albiflora).Fish physiology and biochemistry · 2026Article
- The DNA mismatch repair protein Msh4 is essential for meiosis of male but not for female in zebrafish.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Article
- Germ cell manipulation in farmed fishes.The Journal of reproduction and development · 2026Review
- Genome-Edited Fish in the Field.Current issues in molecular biology · 2025Review
- Primordial germ cell specification and early developmental cell states in Pacific oyster.BMC genomics · 2025Article
- Transcriptome of Sterile Testes inGenes · 2025Article
- Role of multi-omics in aquaculture genetics and breeding: current status and future perspective.Science China. Life sciences · 2025Review
- Advancing genetic improvement in the omics era: status and priorities for United States aquaculture.BMC genomics · 2025Review
- Generation of IgMScientific reports · 2025Article
- The master male sex determinant Gdf6Y of the turquoise killifish arose through allelic neofunctionalization.Nature communications · 2025Article
- In-depth investigation of genome to refine QTL positions for spontaneous sex-reversal in XX rainbow trout.PloS one · 2025Article
- Early fasting does not impact gonadal size nor vasa gene expression in the European seabass Dicentrarchus labrax.Fish physiology and biochemistry · 2024Article
- Chromosomal aberrations and early mortality in a non-mammalian vertebrate: example from pressure-induced triploid Atlantic salmon.Heredity · 2024Article
- Opportunities for CRISPR-Cas9 application in farm animal genetic improvement.Molecular biology reports · 2024Review
- Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla).Fish physiology and biochemistry · 2024Article
- CRISPR/Cas9 Technology for Enhancing Desirable Traits of Fish Species in Aquaculture.International journal of molecular sciences · 2024Review
38 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introgression of farmed salmon escapees into wild stocks is a major threat to the genetic integrity of wild populations. Using germ cell-free fish in aquaculture may mitigate this problem. Our study investigated whether it is possible to produce germ cell-free salmon in F0 by using CRISPR-Cas9 to knock out dnd, a factor required for germ cell survival in vertebrates. To avoid studying mosaic animals, sgRNA targeting alb was simultaneously used as a visual tracer since the phenotype of alb KO is complete loss of pigmentation. Induced mutations for the tracer (alb) and the target (dnd) genes were highly correlated and produced germ cell-less fish lacking pigmentation, underlining the suitability of alb KO to serve as tracer for targeted double allelic mutations in F0 animals in species with prohibitively long generation times. This is also the first report describing dnd knockout in any fish species. Analyzing gene expression and histology of dnd KO fish revealed that sex differentiation of the somatic compartment does not depend on the presence of germ cells. However, the organization of the ovarian somatic compartment seems compromised in mutant fish.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.